Description

• General description: Prolidase is a cytosolic exopeptidase.[1] It is a homodimeric enzyme which requires divalent cations like manganese as a cofactor in its active site for its function.[2]
• Application: Prolidase from porcine kidney has been used:in the enzymatic hydrolysis of porcine milk for the recovery of L-glutamine from proteins and peptides[3]in the proteolysis of skim milk for the determination of ε-(γ-glutamyl)lysine and free aminoacids[4]to determine its effect on the activity of enterococcin A 2000[5]
• Biochem/physiol Actions: Prolidase is an enzyme that catalyzes the hydrolysis of the imide bond between an α-carboxyl group and proline or hydroxyproline. The protein forms a homodimer that hydrolyzes dipeptides or tripeptides with C-terminal proline or hydroxyproline residues.
• Unit Definition: One unit will hydrolyze 1.0 μmole of Gly-Pro per min at pH 8.0 at 40 °C.
• Physical form: Supplied as a lyophilized powder containing Tris buffer salt and MnCl2.