P8415
Peroxidase from horseradish
Type XII, essentially salt-free, lyophilized powder, ≥250 units/mg solid (using pyrogallol)
Manufacturer: Sigma Aldrich
CAS Number: 9003-99-0
Synonym(S): Donor:hydrogen-peroxide oxidoreductase, Horseradish peroxidase
Select a Size
| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| 500 UNITS | P8415-500-UNITS | In Stock | ₹ 8,602.50 |
| 1000 UNITS | P8415-1000-UNITS | In Stock | ₹ 12,820.50 |
| 2000 UNITS | P8415-2000-UNITS | In Stock | ₹ 25,074.90 |
| 5000 UNITS | P8415-5000-UNITS | In Stock | ₹ 44,200.20 |
P8415 - 500 UNITS
In Stock
Quantity
1
Base Price: ₹ 8,602.50
GST (18%): ₹ 1,548.45
Total Price: ₹ 10,150.95
type
Type XII
Quality Level
200
form
essentially salt-free, lyophilized powder
specific activity
≥250 units/mg solid (using pyrogallol)
mol wt
~44 kDa
solubility
0.1 M phosphate buffer: soluble (pH 6.0)H2O: soluble
absorbance ratio
RZ ≥3.0
storage temp.
2-8°C
InChI
1S/H2O3/c1-3-2/h1-2H
InChI key
JSPLKZUTYZBBKA-UHFFFAOYSA-N
Other Options
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Description
- General description: HRP is a single chain polypeptide containing four disulfide bridges. It is a glycoprotein containing 18% carbohydrate. The carbohydrate composition consists of galactose, arabinose, xylose, fucose, mannose, mannosamine, and galactosamine depending upon the specific isozyme. Its molecular weight (~44 kDa) includes the polypeptide chain (33,890 Daltons), hemin plus Ca2+ (~700 Daltons), and carbohydrate (~9,400 Daltons). At least seven isozymes of HRP exist. The isoelectric point for horseradish peroxidase isozymes ranges from 3.0 - 9.0.
- Application: Horseradish peroxidase (HRP) is isolated from horseradish roots (Amoracia rusticana) and belongs to the ferroprotoporphyrin group of peroxidases. It is used in biochemistry applications such as western blots, ELISA and Immunohistochemistry. Horseradish peroxidase is used to amplify a weak signal and increase detectability of a target molecule, such as a protein. Product P8415, type XII, is an essentially salt free lyophilized powder. It is a further purification of product P8375. It is commonly used to determine amounts of glucose and peroxides in solution. It has been used in an aspergillus fumigatus rapid susceptibility assay[1].
- Biochem/physiol Actions: HRP readily combines with hydrogen peroxide (H2O2) and the resultant [HRP-H2O2] complex can oxidize a wide variety of hydrogen donors. The optimal pH is 6.0-6.5 and the enzyme is most stable in the pH range of 5.0-9.0. HRP can be conjugated to antibodies by several different methods including glutaraldehyde, periodate oxidation, through disulfide bonds, and also via amino and thiol directed cross-linkers. It is smaller and more stable than the enzyme labels β-galactosidase and alkaline phosphatase and hence, it is the most desired label. Also, its glycosylation leads to lower non-specific binding.[5] It is also used for the determination of glucose[6] and peroxides[7] in solution.
- Other Notes: A further purification of Peroxidase TypeVI (P8375).
- Unit Definition: One pyrogallol unit will form 1.0 mg purpurogallin from pyrogallol in 20 sec at pH 6.0 at 20 °C.
- Preparation Note: Chromatographically purified
- Analysis Note: Preliminary isoelectric focusing data indicates this is primarily isoenzyme C
SAFETY INFORMATION
Pictograms
Signal Word
Danger
Hazard Statements
Precautionary Statements
Hazard Classifications
Resp. Sens. 1
WGK
WGK 1
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves
Compare Similar Items
Show Difference
type: Type XII
Quality Level: 200
form: essentially salt-free, lyophilized powder
specific activity: ≥250 units/mg solid (using pyrogallol)
mol wt: ~44 kDa
solubility: 0.1 M phosphate buffer: soluble (pH 6.0)H2O: soluble
absorbance ratio: RZ ≥3.0
storage temp.: 2-8°C
InChI: 1S/H2O3/c1-3-2/h1-2H
InChI key: JSPLKZUTYZBBKA-UHFFFAOYSA-N
type:
Type XII
Quality Level:
200
form:
essentially salt-free, lyophilized powder
specific activity:
≥250 units/mg solid (using pyrogallol)
mol wt:
~44 kDa
solubility:
0.1 M phosphate buffer: soluble (pH 6.0)H2O: soluble
absorbance ratio:
RZ ≥3.0
storage temp.:
2-8°C
InChI:
1S/H2O3/c1-3-2/h1-2H
InChI key:
JSPLKZUTYZBBKA-UHFFFAOYSA-N
type: __
Quality Level: 500
form: liquid
specific activity: __
mol wt: __
solubility: __
absorbance ratio: __
storage temp.: 2-8°C
InChI: __
InChI key: __
type:
__
Quality Level:
500
form:
liquid
specific activity:
__
mol wt:
__
solubility:
__
absorbance ratio:
__
storage temp.:
2-8°C
InChI:
__
InChI key:
__
type: __
Quality Level: 200
form: powder
specific activity: __
mol wt: __
solubility: __
absorbance ratio: __
storage temp.: −20°C
InChI: 1S/2C12H27N.H4O7P2/c2*1-4-7-10-13(11-8-5-2)12-9-6-3;1-8(2,3)7-9(4,5)6/h2*4-12H2,1-3H3;(H2,1,2,3)(H2,4,5,6)
InChI key: WRMXOVHLRUVREB-UHFFFAOYSA-N
type:
__
Quality Level:
200
form:
powder
specific activity:
__
mol wt:
__
solubility:
__
absorbance ratio:
__
storage temp.:
−20°C
InChI:
1S/2C12H27N.H4O7P2/c2*1-4-7-10-13(11-8-5-2)12-9-6-3;1-8(2,3)7-9(4,5)6/h2*4-12H2,1-3H3;(H2,1,2,3)(H2,4,5,6)
InChI key:
WRMXOVHLRUVREB-UHFFFAOYSA-N
type: __
Quality Level: 200
form: powder
specific activity: __
mol wt: __
solubility: H2O: 10/150 g/mL, clear
absorbance ratio: __
storage temp.: __
InChI: 1S/2K.H2O4S/c;;1-5(2,3)4/h;;(H2,1,2,3,4)/q2*+1;/p-2
InChI key: OTYBMLCTZGSZBG-UHFFFAOYSA-L
type:
__
Quality Level:
200
form:
powder
specific activity:
__
mol wt:
__
solubility:
H2O: 10/150 g/mL, clear
absorbance ratio:
__
storage temp.:
__
InChI:
1S/2K.H2O4S/c;;1-5(2,3)4/h;;(H2,1,2,3,4)/q2*+1;/p-2
InChI key:
OTYBMLCTZGSZBG-UHFFFAOYSA-L