R1153
RNase B Glycoprotein Standard from bovine pancreas
≥90% (SDS-PAGE), lyophilized powder
Manufacturer: Sigma Aldrich
CAS Number: 9001-99-4
Synonym(S): Ribonuclease B from bovine pancreas, RNase B
Select a Size
| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| 500 μG | R1153-500-μG | In Stock | ₹ 38,150.70 |
| 1 MG | R1153-1-MG | In Stock | ₹ 65,878.50 |
R1153 - 500 μG
In Stock
Quantity
1
Base Price: ₹ 38,150.70
GST (18%): ₹ 6,867.126
Total Price: ₹ 45,017.826
product name
RNase B Glycoprotein Standard from bovine pancreas, Proteomics Grade
grade
Proteomics Grade
Quality Level
200
Assay
≥90% (SDS-PAGE)
form
lyophilized powder
storage temp.
−20°C
InChI
1S/C9H14N4O3/c10-2-1-8(14)13-7(9(15)16)3-6-4-11-5-12-6/h4-5,7H,1-3,10H2,(H,11,12)(H,13,14)(H,15,16)
InChI key
CQOVPNPJLQNMDC-UHFFFAOYSA-N
Other Options
Related Products
Description
- Application: RNase B is a preferred substrate with PNGase F for demonstration of N-linked deglycosylation using SDS-PAGE or MALDI-MS. The activity of PNGase F is routinely assayed using RNase B by monitoring the pronounced mobility shift in 12% gels after deglycosylation. Proteomics Grade RNase B has been used as a source of N-glycans following enzymatic digestions and subsequent purification.[1] It has also been used as a glycoprotein standard.[2]
- Other Notes: Bovine pancreatic RNase B is a glycoprotein that contains only N-linked glycans. It is a globular protein composed of a single domain that occurs naturally as a lesser component in mixture along with ribonuclease A (RNase A), which is the non-glycosylated core form. RNase B contains a single glycosylation site at Asn34 at which from five to nine mannose residues are attached to the chitobiose core, i.e. Man5-9GlcNAc2. Due to the heterogeneity in the glycosylation at Asn34, RNase B exists as five glycosylated varients, with a molecular weight of approx. 15 kDa.
- Quality: RNAse B Glycoprotein Standard has been highly purified to remove contaminating RNase A.
SAFETY INFORMATION
Pictograms
Signal Word
Danger
Hazard Statements
Precautionary Statements
Hazard Classifications
Resp. Sens. 1
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves