Chondroitinase ABC from Proteus vulgaris

Description

• General description: Chondroitinase ABC is a lyase with specificity toward chondroitin sulfates and dermatan sulfates - with some minor activity toward hyaluronan. This recombinant version only contains the endolytic chondroitinase, meaning it will not digest the non-reducing ends of proteoglycan substrates. It targets β-(1,4)-linkages between N-acetylgalactosamine and either D-glucuronic acid or L-iduronic acid. The product of digestion is a mixture of Δ 4-unsaturated tetra- and di-saccharides.
• Application: Degradation of Chondroitin sulfate-containing tissue such as extracellular matrices and cartilageNeural regeneration researchEliminative degradation of all forms of Chondroitin sulfate: A, B and CQuantitation of glucosaminoglycans (mucopolysaccharides)
• Features and Benefits: Low EndotoxinBSA FreeRecombinantEndolytic component only, does not contain exolytic enzyme.Compatible with chondroitin 4-sulfate, chondroitin 6-sulfate, and dermatan sulfate, and acts slowly on hyaluronate
• Components: Non active Ingredients: Hepes-Sodium salt, NaCl, Trehalose, Imidazole, Glycerol, Water
• Compatibility: pH optimum: pH 8.0 (chondroitin sulfate) pH 6.8 (hyaluronic acid)Temperature optimum: 37 °CActivator: 0.05 M acetate Inhibitors: 1 mM Zn2+ Ni2+, Fe2+ and Cu2+
• Unit Definition: 1 unit will liberate 1.0 μmole of unsaturated disaccharides per min at pH 8.0 at 37 °C, using chondroitin sulfate from shark cartilage (C4384) as substrate.
• Storage and Stability: Store the product at -70 °C. When stored unopened at -70 °C, the buffer is stable for at least two years.
• Other Notes: This product is for R&D use only. Not for drug, household, or other uses. Please consult the SafetyData Sheet for information regarding hazards and safe handling practices.