Trypsin Sequencing Grade, modified

Description

• General description: Trypsin Sequencing Grade, modified, is isolated from bovine pancreas as a highly purified and specific protease, and subsequently modified.Trypsin is a highly efficient and specific protease widely used in proteomics for protein digestion. It produces short peptides with specific characteristics that are compatible with current separation and identification methods[1] such as liquid chromatography, mass spectrometry (MS).[2][3]Inhibitors: TLCK, DFP, PMSF, leupeptin, soybean trypsin inhibitor, trypsin inhibitor from hen egg, aprotinin, α2-macroglobulin,α1-antitrypsin, APMSF, and antipain.
• Specificity: Trypsin is a serine endopeptidase. At pH 7.5–9, it specifically hydrolyzes proteins and peptide bonds C-terminally of Iysine and arginine. Amide and ester bonds of Arg and Lys are also cleaved. The specificity of Trypsin Sequencing Grade, modified, is verified with the oxidized B-chain of insulin (insulin Box) as a substrate. High concentrations of Trypsin Sequencing Grade, modified, one part by weight enzyme with 9 parts by weight insulin Box, are incubated for 18 hours to detect traces ofchymotrypsin impurities.
• Application: Use Trypsin Sequencing Grade, modified, to generate glycopeptides from purified glycoproteins.It is used for:Protein-structure elucidationTryptic mappingFingerprinting analysisSequence analysisTranslocation studiesProtein identification[4][5][6][7]Protein digestion during lipoprotein preparation for liquid chromatography-tandem mass spectrometry (LC-MS/MS)[8]
• Quality: Purity: Free of impurities that may interfere with the separation of peptides in reversed-phase HPLC.
• Preparation Note: Working concentration: 1/100 to 1/5 of the protein by weightStorage conditions (working solution): -15 to -25 °CTrypsin Sequencing Grade, modified, is more resistant to autolysis, even at pH values in the neutral and weakly basic range. The enzyme can be used in high concentrations.A solution in 1% acetic acid or 1 mM HCI can be used for up to one week when stored at 2 to 8° C. Stored in aliquots at -15 to -25 °C, the solution is stable for at least one year without loss of activity.
• Storage and Stability: Store dry
• Other Notes: For life science research only. Not for use in diagnostic procedures.