Description

• Application: Pepsin cleavage can be used to produce F(ab′)2 fragments of antibodies. pepsin at www.sigma-aldrich.com/enzymeexplorer.
• Biochem/physiol Actions: Preferential cleavage: hydrophobic and aromatic residues in P1 and P1′ postitions. Cleaves Phe-Val, Gln-His, Glu-Ala, Ala-Leu, Leu-Tyr, Tyr-Leu, Gly-Phe, Phe-Phe and Phe-Tyr bonds in the β chain of insulin
• Unit Definition: One unit will produce a change in A280 of 0.001 per minute at pH 2.0 at 37 deg C, measured as TCA-Soluble products using hemoglobin as substrate.
• Analysis Note: Optimum pH is 2-4. Active in 4 M urea and 3 M guanidine HCl. Stable at 60 °C. Pepsin is irreversibly inactivated at pH 8.0 - 8.5.
• Legal Information: Vetec is a trademark of Merck KGaA, Darmstadt, Germany