Invitrogen™ LentiArray™ Human Ubiquitin CRISPR Library

Description

• The award-winning Invitrogen™ LentiArray™ Human Ubiquitin Library targets 943 genes with up to 4 gRNA per gene target (pooled in a single well) for a total of 3,722 gRNAs
• Libraries are delivered as 100 μL of ready to use lentiviral particles per gene target with an average titer of 1x10 8 TU/mL (functional titer determined by antibiotic resistance)
• The ubiquitin system is integral to maintaining cellular homeostasis by regulating protein turnover
• Dysregulation of the ubiquitin system has been linked to multiple diseases, including cancer, neurodegenerative, musculoskeletal, cardiovascular, and metabolic diseases, as well as being linked to viral infection
• The gene targets within this library were selected using the most up-to-date genome databases, including the NCBI RefSeq database, and cross-referenced to the Gene Ontology Consortium (GO) database and/or the HUGO Gene Nomenclature Committee (HGNC)
• Built to bring you success LentiArray CRISPR libraries are constructed using gRNA designs produced using Thermo Fisher Scientific’s proprietary CRISPR gRNA design algorithm
• This algorithm incorporates the latest gRNA design research, as well as our extensive in-house experience, to produce the highest quality designs
• The gRNA designs included in the LentiArray CRISPR libraries are selected for maximal editing efficiency and specificity and are designed to knock out all known isoforms of the target gene
• For each gene target, up to four high-quality gRNAs are included to help ensure highly efficient knockout of the target gene in a wide array of cell types
• Design and execute your experiments without limitations LentiArray CRISPR libraries are designed and constructed to give you complete control over your experimental design
• They utilize a two-vector design, expressing the Cas9 nuclease and the gRNA off of separate lentiviral constructs, enabling you to dictate when and how the genome editing tools are delivered to your cells
• The LentiArray Cas9 lentivirus construct expresses a human, codon-optimized Cas9 nuclease with a blasticidin-resistance gene under the control of the EF-1α (EFS) promoter
• You can choose to co-infect your cells with both the LentiArray gRNA lentiviruses and the Cas9 lentivirus or just the LentiArray Cas9 lentivirus to establish a stable Cas9 expressing cell line
• With LentiArray CRISPR libraries you have the freedom to design the experimental approach that best suits your model system and your screening goals
• Furthermore, to minimize restrictions to your experimental design, the gRNA lentiviruses that make up the LentiArray CRISPR libraries do not contain fluorescent markers
• This allows reporters of any wavelength to be used with the libraries and expands your ability to perform multiplexed experiments
• For challenging cell lines, the LentiArray gRNA lentiviruses contain a puromycin resistance gene that allows enrichment of the population of edited cells and drives stronger phenotypes
• Optimize your assay design and have full confidence in your results with LentiArray controls High quality controls are the cornerstone of a successful screen
• The LentiArray CRISPR libraries product line includes a suite of controls to help you quickly develop your assay and give you confidence in defining your hit criteria.