Thermo Scientific™ Pierce™ Recombinant Protein G

Description

• Purified and conjugated forms of Thermo Scientific™ Pierce Recombinant Protein G enable probing and detection of mouse and human antibodies, especially IgG isotypes, in Western blotting, ELISA, IHC and other immunoassay protocols
• Protein G is a bacterial cell wall protein isolated from group G Streptococci
• DNA sequencing of native Protein G identifies two IgG-binding domains and sites for albumin and cell surface binding
• The albumin and cell surface binding domains have been eliminated from Recombinant Protein G to reduce nonspecific binding and, therefore, can be used to separate IgG from crude samples
• Optimal binding occurs at pH 5, although binding is also effective at pH 7.0 to 7.2
• Highlights: Contains two Fc-binding domains per protein Better than Protein A for mouse (incl
• IgG1), human (incl
• IgG3) and rat, goat and cow antibodies Poorer than Protein A for guinea pig, pig, dog and cat Does not bind human IgM, IgD or IgA