4532077

Thermo Scientific™ Restore™ PLUS Western Blot Stripping Buffer

Remove high-affinity antibodies from membranes so they can be reprobed and detected with chemiluminescent substrates.

Manufacturer: Fischer Scientific

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Form

Liquid

Product Line

Restore™

Content And Storage

Upon receipt store at room temperature. Product shipped at ambient temperature.

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Description

  • Thermo Scientific™ Restore PLUS Western Blot Stripping Buffer is an advanced formula for removing bound primary and secondary antibodies from membranes so they can be reprobed and detected with chemiluminescent substrates
  • Restore PLUS Buffer is an alternative formulation of the original Thermo Scientific Restore Western Blot Stripping Buffer
  • Restore PLUS Stripping Buffer was designed for use with antibodies that are difficult to remove from Western blots and require longer incubation times or incubation temperatures greater than 22°C with gentler formulations
  • High-affinity antibodies can be quickly and effectively stripped from Western blots at room temperature without removing transferred proteins, thereby allowing multiple reprobes of the target
  • Highlights: Ready and easy to use – no dilution necessary; no offensive odors; store at room temperature Compatible – use on nitrocellulose and PVDF membranes, whether still wet or already dry; works with practically any blocking buffer, enzyme conjugate and chemiluminescent substrate Cost effective – save valuable time and samples; strip blots effectively the first time Robust yet gentle – transferred proteins remain viable; strip the same blot up to five times Flexible – strip and reprobe to optimize antibody concentrations or to detect a new antigen with different antibodies

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