Thermo Scientific™ Pierce™ Western Blot Signal Enhancer

Description

• Thermo Scientific™ Pierce Western Blot Signal Enhancer is a two-reagent system for conditioning protein blots after transfer to greatly enhance the effectiveness of primary antibodies and intensify the final detection signal in Western blot experiments
• The Pierce Western Blot Signal Enhancer membrane treatment procedure is very simple, takes only 15 minutes and can be added to nearly any existing Western blotting protocol
• The result is an increase in the intensity of target protein bands on the Western blot or detection of target proteins at levels that were previously not possible
• The product is effective for signal intensification with both chemiluminescent and chromogenic substrates, especially with nitrocellulose membranes
• Highlights: Increases protein detection – most protein targets show a three- to 10-fold increase in signal intensity, enabling much less protein to be detected with the same substrate and method Improves antibody binding – the membrane-treatment reagent exposes and conditions target proteins so that specific antibodies can bind more effectively Works for nearly any protein – signal enhancement has been demonstrated with targets such as IL-6, p53, NFkB, BRCA1 and EGF Effective with any substrate – enhances both chemiluminescent (ECL) and colorimetric detection for Western blots Compatible with any membrane – enhances signal on nitrocellulose and PVDF membrane, regardless of pore size (enhancement is less pronounced with PVDF) Fast, 15-minute protocol – optimized for a combination of simplicity, speed and signal enhancement for most proteins Ready-to-use – no formulating or diluting necessary, and the reagents are stable for storage at room temperature Includes: Enhancer Reagent 1, 250mL; Enhancer Reagent 2, 250mL