Thermo Scientific™ Pierce™ Fast Blocking Buffer

Description

• Optimize Western blotting systems by decreasing both background signal and incubation time compared to traditional blocking agents with Thermo Scientific™ Pierce Fast Blocking Buffer, a ready-to-use blocker solution
• Additional Information and Online Ordering Pierce Fast Blocking Buffer streamlines Western blot protocols by providing consistent, high-quality blocking for nitrocellulose or PVDF membranes in only 5 minutes
• Blocking and probing steps with Fast Blocking Buffer prevents nonspecific binding of detection antibodies during probing steps but allows specific detection to occur
• Pierce Fast Wash Buffer provides efficient membrane washing after incubation with a primary and secondary antibody to effectively shorten total washing time to 20 minutes (from the typical 90 minutes)
• These two buffers are compatible with antibodies and biotin-avidin systems, as well as both nitrocellulose and PVDF membranes
• The blocking buffer provides rapid and effective blocking in just five minutes while producing results comparable to traditional blockers for Western blots
• Highlights: Fast – use both buffers to shorten the typical Western blot development by over 2 hours Simple – optimized protocol makes Western blot analysis easier than ever Low background – provides results comparable to classic Western blotting buffers