Thermo Scientific™ Pierce™ Far-Western Blot Kit for Biotinylated Proteins

Description

• Thermo Scientific™ Pierce Far-Western Blot Kit for Biotinylated Proteins enables in-gel or on-membrane Western blot detection of target proteins via interaction with biotin-labeled test proteins as primary probes
• This far-Western blotting kit provides the reagents and protocol necessary to probe for target proteins by virtue of their interaction with a specific binding partner, which the researcher supplies in purified, biotinylated form
• The kit is successful for analysis of protein-protein interactions in which the target (prey) proteins can be bound after they have been separated by gel electrophoresis
• The kit includes reagents and procedures for probing and chemiluminescent detection with blots or directly from mini gels
• Highlights: On-membrane or in-gel detection options – on-membrane detection offers greater sensitivity; in-gel detection is faster and prevents problems associated with incomplete or inefficient transfer Nonradioactive alternative for far-Western analysis – reliable and sensitive biotin/streptavidin-HRP chemistry combined with chemiluminescent detection offers a practical and safe alternative to radiolabeling the bait protein Useful interaction range – effective for interaction pairs that have moderate to strong binding associations between the prey and the biotinylated bait protein Primary antibody-free detection – uses a biotinylated protein as the probe, eliminating the need for primary antibodies or even to have prior knowledge of target-protein identities Compatible with both SDS-PAGE and native gels – provides option to probe for prey proteins in a more native environment because reduced or denaturing systems may not always allow for the intended interaction Reduced nonspecific binding – biotin/streptavidin-HRP systems demonstrate less nonspecific binding compared to antibodies directed against the bait protein Compatible with protein staining – gels can be used for total protein staining after the in-gel chemiluminescent detection step, eliminating the need to run two gels Includes: Streptavidin-HRP, dilution buffer, PBS Packs, stable peroxide and luminol enhancer chemiluminescent substrate, detergent solution and pre-cut cellophane sheets