Nitrocellulose Unsupported Membrane (EP) Highest sensitivity for protein blotting and immunoblotting, or other procedures requiring optimum resolution Easily blocked Wets out naturally and does not require methanol like PVDF membranes Nitrocellulose Supported Membrane (WP) High sensitivity but with added strength of a polyester matrix Surfactant-free membranes have no additives to interfere with binding capacity