Invitrogen™ iBlot™ 3 Transfer Stacks, midi, nitrocellulose

Description

• iBlot 3 Transfer Stacks are used with the iBlot 3 Western Blot Transfer Device for the transfer of proteins from an SDS-PAGE gel to a membrane support when performing western blot experiments
• iBlot 3 Transfer Stacks contain copper-fiber electrode sheets, optimized cathode and anode solid buffer gels, and a preconditioned nitrocellulose transfer membrane
• They are compatible with common commercially available protein gels, including Bis-Tris, Tris-Glycine, Tris-Acetate, and Tricine chemistries
• This midi-size stack (13 x 8.3 cm) can be used with midi-size gels or two mini-size gels
• The iBlot 3 Western Blot Transfer System (iBlot 3 Transfer Stacks plus iBlot 3 Transfer Device) has been optimized to provide protein transfers in 3–8 minutes that produce higher quality and more reliable western blot result compared to many other transfer methods
• Nitrocellulose membrane The nitrocellulose membrane (0.2-μm pore size) in this stack is composed of 100% pure nitrocellulose to provide a high-quality transfer
• The membrane is compatible with commonly used detection methods such as staining, immunodetection, fluorescence, and radiolabeling
• Proteins bind to the membrane due to hydrophobic and electrostatic interactions
• The protein-binding capacity is 209 μg/cm 2 .