Fisherbrand™ Semidry Blotting Apparatus

Description

• Transfers minigels in 15 to 30 minutes and full-sized gels in 30 to 60 minutes, with efficiencies of nearly 90%
• Multiple gels can be transferred by stacking gel/membrane/filter paper “sandwiches” Because plate electrodes are in direct contact with “sandwich,” a homogeneous electric field is created, ensuring even transfers System requires only minimal amounts of buffer (about 250mL) Low voltage—requires less than 1mA/cm 2 of current; no external cooling is necessary Features 20 × 20cm platinum-coated titanium anode and stainless-steel cathode Transfers proteins from polyacrylamide electrophoresis gels onto hybridization membranes (Western transfers) Ideal for membrane-based, solid-phase protein assays such as HIV testing, where viral antigens are assayed with patient antibodies Use to detect trace proteins in complex mixtures and study protein interaction with other molecules (i.e., DNA)