Thermo Scientific™ Pierce™ ECL Plus Western Blotting Substrate

Description

• Thermo Scientific™ Pierce ECL Plus Substrate is an acridan-based chemiluminescent and chemifluorescent substrate to detect peroxidase activity from HRP-conjugated probes by Western blotting using X-ray film, CCD or laser-based imagers
• Pierce ECL Plus Substrate is a chemiluminescent and chemifluorescent substrate to detect peroxidase activity from HRP-conjugated antibodies and probes by Western blotting using X-ray film, CCD camera documentation systems or laser-based imagers
• Pierce ECL Plus Substrate generates acridinium esters when it reacts with HRP
• As these ester intermediates react with peroxide, they produce a strong and sustained chemiluminescent signal that can be captured by X-ray film CCD imaging
• The substrate also produces a robust fluorescent signal that can be captured with fluorescence imagers
• Pierce ECL Plus Substrate enables the detection of low-picogram amounts of target protein on nitrocellulose or PVDF membrane when probed with appropriate primary and secondary antibody concentrations
• Its broad dynamic range, high sensitivity and long-lasting signal make Pierce ECL Plus Substrate an excellent choice for Western blotting
• Highlights: Easy to use — can be substituted for the discontinued GE ECL Plus Substrate without any re-optimization Higher sensitivity — detect targets down to the low-picogram level Longer signal duration — sustained light output for as long as five hours More imaging options — X-ray, CCD or laser-based imagers More affordable — high quality and performance at a lower price than other suppliers« similar substrates