Thermo Scientific™ SuperSignal™ West Pico PLUS Chemiluminescent Substrate

Description

• Thermo Scientific SuperSignal West Pico PLUS Chemiluminescent Substrate is an enhanced chemiluminescent (ECL) horseradish peroxidase (HRP) substrate that enables picogram to high femtogram-level protein detection by western blot analysis
• SuperSignal West Pico PLUS Chemiluminescent Substrate characteristics include: Sensitivity: pictogram to femtogram sensitivity Stability: 8-hr working solution stability; 1-year kit stability at room temperature Compatibility: nitrocellulose and PVDF membranes Signal duration: 6 to 24 hours Recommended primary antibody concentration: 1:1000–1:5000 dilution (0.2–1.0 μg/mL) Recommended secondary antibody concentration: 1:20,000–100,000 dilution (10–50 ng/mL) SuperSignal West Pico PLUS substrate is designed to provide excellent signal intensity and sensitivity for western blotting with HRP conjugates
• The intensity of the light emission combined with the exceptional duration allows for the acquisition of multiple exposures to more easily obtain publication-quality blot images
• SuperSignal West Pico PLUS substrate is compatible with different membranes, blocking reagents, and a wide range of antibody dilutions, making it an ideal choice for most western blotting applications.