PI34094
Thermo Scientific™ SuperSignal™ West Femto Maximum Sensitivity Substrate
Manufacturer: Fischer Scientific
Select a Size
| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| Each of 1 | PI34094-Each-of-1 | In Stock | ₹ 14,613.65 |
PI34094 - Each of 1
In Stock
Quantity
1
Base Price: ₹ 14,613.65
GST (18%): ₹ 2,630.457
Total Price: ₹ 17,244.107
Product Type
Substrate
Form
Liquid
Membrane Compatibility
Nitrocellulose, PVDF
Quantity
20 mL
Recommended Antibody Concentrations
2°: 1:100-500K (2-10ng/mL), 1°: 1:5K (10- 200ng/mL)
Signal Duration
6 to 8 hr.
Kit Contents
Stable Peroxide Buffer (10 mL), Luminol/Enhancer (10 mL)
Detection Method
Chemiluminescence
For Use With (Application)
Western Blot
Product Line
SuperSignal™
Substrate
Horseradish Peroxidase
Sensitivity
Low femtogram-level
Sufficient For
2 Mini-Gel Blots, 200 sq. cm of Membrane
Content And Storage
Sufficient For: 2 mini-gel blots; 200 cm2of membrane• Luminol/Enhancer, 10 mL• Stable Peroxide Buffer, 10 mLStore reagents at 4°C.
Related Products
Description
- Thermo Scientific™ SuperSignal West Femto Substrate is an ultra-sensitive enhanced chemiluminescent substrate (ECL substrate) for detection of peroxidase activity from HRP-conjugated antibodies and probes in Western blotting
- SuperSignal West Femto Substrate Maximum Sensitivity Substrate is an ultra-sensitive chemiluminescent substrate system for Western blotting with horseradish peroxidase (HRP) enzyme
- The substrate kit enables detection of low femtogram (that's mid-zeptomole!) amounts of protein deposited on nitrocellulose or PVDF membrane and probed with appropriate primary and secondary antibodies
- When combined with optimized antibody concentrations and blocking buffers, SuperSignal West Femto Substrate enables detection of target proteins in amounts that are small to be seen with typical ECL substrates
- Highlights: ECL substrate – an enhanced chemiluminescent substrate for detection of horseradish peroxidase (HRP) activity from antibodies and other Western blot probes Sensitive – detect low-femtogram (mid-zeptomole) amounts of protein in bands on nitrocellulose or PVDF membranes Quantitative – produces quantitative signal that is measurable over two orders of magnitude Saves on antibody – optimized for very dilute antibody concentrations; dilute primary antibodies 5000- to 100,000-fold and secondary antibodies 100,000 to 500,000-fold (from 1mg/mL stocks) Intense signal – easy to capture an image by exposure to autoradiography film or via a CCD camera or similar imaging system Excellent signal duration – 8 hours of usable light-output when conditions are optimized Stable reagent – kit components are stable for 1 year at 4°C or 6 months at room temperature
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Product Type: Substrate
Form: Liquid
Membrane Compatibility: Nitrocellulose, PVDF
Quantity: 20 mL
Recommended Antibody Concentrations: 2°: 1:100-500K (2-10ng/mL), 1°: 1:5K (10- 200ng/mL)
Signal Duration: 6 to 8 hr.
Kit Contents: Stable Peroxide Buffer (10 mL), Luminol/Enhancer (10 mL)
Detection Method: Chemiluminescence
For Use With (Application): Western Blot
Product Line: SuperSignal™
Substrate: Horseradish Peroxidase
Sensitivity: Low femtogram-level
Sufficient For: 2 Mini-Gel Blots, 200 sq. cm of Membrane
Content And Storage: Sufficient For: 2 mini-gel blots; 200 cm2of membrane• Luminol/Enhancer, 10 mL• Stable Peroxide Buffer, 10 mLStore reagents at 4°C.
Product Type:
Substrate
Form:
Liquid
Membrane Compatibility:
Nitrocellulose, PVDF
Quantity:
20 mL
Recommended Antibody Concentrations:
2°: 1:100-500K (2-10ng/mL), 1°: 1:5K (10- 200ng/mL)
Signal Duration:
6 to 8 hr.
Kit Contents:
Stable Peroxide Buffer (10 mL), Luminol/Enhancer (10 mL)
Detection Method:
Chemiluminescence
For Use With (Application):
Western Blot
Product Line:
SuperSignal™
Substrate:
Horseradish Peroxidase
Sensitivity:
Low femtogram-level
Sufficient For:
2 Mini-Gel Blots, 200 sq. cm of Membrane
Content And Storage:
Sufficient For: 2 mini-gel blots; 200 cm2of membrane• Luminol/Enhancer, 10 mL• Stable Peroxide Buffer, 10 mLStore reagents at 4°C.
Product Type: Substrate
Form: Liquid
Membrane Compatibility: Nitrocellulose, PVDF
Quantity: 100 mL
Recommended Antibody Concentrations: 2°: 1:100-500K (2-10ng/mL), 1°: 1:5K (10- 200ng/mL)
Signal Duration: 6 to 8 hr.
Kit Contents: Luminol/Enhancer (50 mL), Stable Peroxide Buffer (50 mL)
Detection Method: Chemiluminescence
For Use With (Application): Western Blot
Product Line: SuperSignal™
Substrate: Horseradish Peroxidase
Sensitivity: Low femtogram-level
Sufficient For: 12 Mini-Gel Blots, 1000 sq. cm of Membrane
Content And Storage: Sufficient For: 12 mini-gel blots; 1000 cm2of membrane• Luminol/Enhancer, 50 mL• Stable Peroxide Buffer, 50 mLStore reagents at 4°C.
Product Type:
Substrate
Form:
Liquid
Membrane Compatibility:
Nitrocellulose, PVDF
Quantity:
100 mL
Recommended Antibody Concentrations:
2°: 1:100-500K (2-10ng/mL), 1°: 1:5K (10- 200ng/mL)
Signal Duration:
6 to 8 hr.
Kit Contents:
Luminol/Enhancer (50 mL), Stable Peroxide Buffer (50 mL)
Detection Method:
Chemiluminescence
For Use With (Application):
Western Blot
Product Line:
SuperSignal™
Substrate:
Horseradish Peroxidase
Sensitivity:
Low femtogram-level
Sufficient For:
12 Mini-Gel Blots, 1000 sq. cm of Membrane
Content And Storage:
Sufficient For: 12 mini-gel blots; 1000 cm2of membrane• Luminol/Enhancer, 50 mL• Stable Peroxide Buffer, 50 mLStore reagents at 4°C.
Product Type: Substrate
Form: Liquid
Membrane Compatibility: Nitrocellulose, PVDF
Quantity: 200 mL
Recommended Antibody Concentrations: 2°: 1:100-500K (2-10ng/mL), 1°: 1:5K (10- 200ng/mL)
Signal Duration: 6 to 8 hr.
Kit Contents: Stable Peroxide Buffer (100 mL), Luminol/Enhancer (100 mL)
Detection Method: Chemiluminescence
For Use With (Application): Western Blot
Product Line: SuperSignal™
Substrate: Horseradish Peroxidase
Sensitivity: Low femtogram-level
Sufficient For: 25 mini-gel blots, 2000 sq. cm of membrane
Content And Storage: Sufficient For: 25 mini-gel blots; 2000 cm2of membrane• Luminol/Enhancer, 100 mL• Stable Peroxide Buffer, 100 mLStore reagents at 4°C.
Product Type:
Substrate
Form:
Liquid
Membrane Compatibility:
Nitrocellulose, PVDF
Quantity:
200 mL
Recommended Antibody Concentrations:
2°: 1:100-500K (2-10ng/mL), 1°: 1:5K (10- 200ng/mL)
Signal Duration:
6 to 8 hr.
Kit Contents:
Stable Peroxide Buffer (100 mL), Luminol/Enhancer (100 mL)
Detection Method:
Chemiluminescence
For Use With (Application):
Western Blot
Product Line:
SuperSignal™
Substrate:
Horseradish Peroxidase
Sensitivity:
Low femtogram-level
Sufficient For:
25 mini-gel blots, 2000 sq. cm of membrane
Content And Storage:
Sufficient For: 25 mini-gel blots; 2000 cm2of membrane• Luminol/Enhancer, 100 mL• Stable Peroxide Buffer, 100 mLStore reagents at 4°C.
Product Type: Substrate
Form: Liquid
Membrane Compatibility: Nitrocellulose, PVDF
Quantity: 200 mL
Recommended Antibody Concentrations: 2°: 1:20-100 K (10-50 ng/mL), 1°: 1:1 K (0.2-1 μg/mL)
Signal Duration: 6 to 24 hr.
Kit Contents: SuperSignal West Pico PLUS Luminol/Enhancer, SuperSignal West Pico PLUS Stable Peroxide
Detection Method: Chemiluminescence
For Use With (Application): Western Blot
Product Line: SuperSignal™
Substrate: Horseradish Peroxidase
Sensitivity: Picogram to high femtogram-level
Sufficient For: 25 Mini-Gel Blots, 2000 cm2of Membrane
Content And Storage: Sufficient for 25 mini-gel blots; 2000 cm2of membrane• SuperSignal West Pico PLUS Luminol/Enhancer, 100 mL• SuperSignal West Pico PLUS Stable Peroxide, 100 mLStore at room temperature.
Product Type:
Substrate
Form:
Liquid
Membrane Compatibility:
Nitrocellulose, PVDF
Quantity:
200 mL
Recommended Antibody Concentrations:
2°: 1:20-100 K (10-50 ng/mL), 1°: 1:1 K (0.2-1 μg/mL)
Signal Duration:
6 to 24 hr.
Kit Contents:
SuperSignal West Pico PLUS Luminol/Enhancer, SuperSignal West Pico PLUS Stable Peroxide
Detection Method:
Chemiluminescence
For Use With (Application):
Western Blot
Product Line:
SuperSignal™
Substrate:
Horseradish Peroxidase
Sensitivity:
Picogram to high femtogram-level
Sufficient For:
25 Mini-Gel Blots, 2000 cm2of Membrane
Content And Storage:
Sufficient for 25 mini-gel blots; 2000 cm2of membrane• SuperSignal West Pico PLUS Luminol/Enhancer, 100 mL• SuperSignal West Pico PLUS Stable Peroxide, 100 mLStore at room temperature.