Thermo Scientific™ Pierce™ ECL 2 Western Blotting Substrate

Description

• Pierce ECL 2 Substrate is a chemiluminescent and chemifluorescent substrate to detect peroxidase activity from HRP-conjugated antibodies and probes by Western blotting using X-ray film, CCD camera documentation systems or laser-based imagers
• Pierce ECL 2 Substrate generates acridinium esters when it reacts with HRP
• As these ester intermediates react with peroxide, they produce a strong and sustained chemiluminescent signal that can be captured by X-ray film CCD imaging
• The substrate also produces a robust fluorescent signal that can be captured with fluorescence imagers
• Pierce ECL 2 substrate enables the detection of low-picogram amounts of target protein on nitrocellulose or PVDF membrane when probed with appropriate primary and secondary antibody concentrations
• A broad dynamic range and excellent sensitivity combined with a long-lasting signal make Pierce ECL 2 Substrate a desirable Western blotting substrate
• Highlights: High sensitivity — detect targets down to the low-picogram level Long signal duration — sustained light output for as long as five hours More imaging options — X-ray, CCD or laser-based imagers More affordable — high quality and performance at a lower price than other suppliers' similar substrates