Atto 565 NHS ester

Description

• General description: Atto 565 NHS ester is a commonly used amine-reactive reagent, provided by ATTO-TEC. It is widely used in protein labeling and readily reacts with compounds containing amino groups, forming a chemically stable amide bond between the dye, e.g., a protein. The optimum pH-range for NHS-ester coupling is pH 8.0 – 9.0. At this pH amino groups of proteins, i.e., the ε-amino groups of lysines are unprotonated to a degree sufficiently high for fast coupling.
• Features and Benefits: Strong absorptionHigh fluorescence quantum yieldHigh thermal and photo-stability
• Other Notes: Fluorescence of bioconjugates[1][2]; employed in fluorescence resonance energy transfer, FRET, experiments[3].
• Legal Information: This product is for Research use only. In case of intended commercialization, please contact the IP-holder (ATTO-TEC GmbH, Germany) for licensing.