17-100-99MI

MilliporeSigma™ Upstate™ Acetyl-Histone H4 (Lys8), Rabbbit Polyclonal, ChIP Validated Antibody and Primer Set

Manufacturer: MilliporeSigma™

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Pack Size SKU Availability Price
Each of 1 17-100-99MI-Each-of-1 In Stock ₹ 52,449.99

17-100-99MI - Each of 1

₹ 52,449.99

In Stock

Quantity

1

Base Price: ₹ 52,449.99

GST (18%): ₹ 9,440.998

Total Price: ₹ 61,890.988

Antigen

Acetyl-Histone H4 (Lys8)

Classification

Polyclonal

Formulation

Anti-Acetyl-Histone H4 (Lys8) (rabbit polyclonal). One vial containing 50μL of antiserum containing 0.05% sodium azide. Normal Rabbit Serum. One vial containing 100μL of antiserum containing 0.05% sodium azide. ChIP Primers, HSPCA. One vial containing 75μL of 5μM of each primer specific for human HSPCA. FOR: GCA ACA GCT ACC ACA GGA CCA; REV: GAG CGT GTG AAA TCA ACA TAA AGC

Gene Symbols

HIST1H4A, H4/J, HIST1H4F, HIST1H4L, H4FN, H4FA, H4FH, HIST1H4H, H4F2, H4FM, H4/A, H4FK, H4FG, HIST2H4, H4FB, H4/K, HIST1H4I, H4/N, HIST1H4B, H4FE, H4/M, H4/a, HIST1H4E, HIST4H4, HIST2H4A, H4FC, H4FI, H4/H, HIST1H4C, H4/G, HIST1H4K, H4FJ, H4FD, H4/I, H4/B, H4/D, H4/C, HIST1H4J, HIST1H4D, H4/E

Immunogen

Synthetic peptide corresponding to yeast Histone H4 acetylated at lysine 8.

Quantity

25 Assays

Research Discipline

Epigenetics and Nuclear Function

Gene ID (Entrez)

NM_175054

Includes

The ChIPAb+ Acetyl-Histone H4 (Lys8) set includes the Acetyl-Histone H4 (Lys8) antibody, a negative control rabbit serum and qPCR primers which amplify a 134bp region of human HSPCA.

Content And Storage

-20°C for up to 12 months from date of receipt; Upon first thaw and prior to removing the cap centrifuge the vial and gently mix the solution; Aliquot into microcentrifuge tubes and store at −20°C; Avoid repeated freeze/thaw cycles

Applications

ChIP Assay, Immunoprecipitation, Western Blot

Conjugate

Unconjugated

Gene Accession No.

P62805

Host Species

Rabbit

Purification Method

Unpurified

Regulatory Status

RUO

Primary or Secondary

Primary

Test Specificity

Recognizes acetyl-Histone H4 (Lys8).

Target Species

Human, S. cerevisiae, Yeast

Form

Serum

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Description

  • Specifically detects Acetyl-Histone H4 (Lys8) Clone: in Human, S
  • cerevisiae, Yeast samples, and it is validated for Chromatin Immunoprecipitation, Immunoprecipitation, Western Blotting Histone H4 is one of the 5 main histone proteins involved in the structure of chromatin in eukaryotic cells
  • Featuring a main globular domain and a long N terminal tail H4 is involved with the structure of the nucleosomes of the 'beads on a string' structure
  • The N-terminal tail of histone H4 protrudes from the globular nucleosome core and can undergo several different types of epigenetic modifications that influence cellular processes
  • These modifications include the covalent attachment of methyl or acetyl groups to lysine and arginine amino acids and the phosphorylation of serine or threonine.

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MilliporeSigma™

17-100-99MI

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Antigen:
Acetyl-Histone H4 (Lys8)

Classification:
Polyclonal

Formulation:
Anti-Acetyl-Histone H4 (Lys8) (rabbit polyclonal). One vial containing 50μL of antiserum containing 0.05% sodium azide. Normal Rabbit Serum. One vial containing 100μL of antiserum containing 0.05% sodium azide. ChIP Primers, HSPCA. One vial containing 75μL of 5μM of each primer specific for human HSPCA. FOR: GCA ACA GCT ACC ACA GGA CCA; REV: GAG CGT GTG AAA TCA ACA TAA AGC

Gene Symbols:
HIST1H4A, H4/J, HIST1H4F, HIST1H4L, H4FN, H4FA, H4FH, HIST1H4H, H4F2, H4FM, H4/A, H4FK, H4FG, HIST2H4, H4FB, H4/K, HIST1H4I, H4/N, HIST1H4B, H4FE, H4/M, H4/a, HIST1H4E, HIST4H4, HIST2H4A, H4FC, H4FI, H4/H, HIST1H4C, H4/G, HIST1H4K, H4FJ, H4FD, H4/I, H4/B, H4/D, H4/C, HIST1H4J, HIST1H4D, H4/E

Immunogen:
Synthetic peptide corresponding to yeast Histone H4 acetylated at lysine 8.

Quantity:
25 Assays

Research Discipline:
Epigenetics and Nuclear Function

Gene ID (Entrez):
NM_175054

Includes:
The ChIPAb+ Acetyl-Histone H4 (Lys8) set includes the Acetyl-Histone H4 (Lys8) antibody, a negative control rabbit serum and qPCR primers which amplify a 134bp region of human HSPCA.

Content And Storage:
-20°C for up to 12 months from date of receipt; Upon first thaw and prior to removing the cap centrifuge the vial and gently mix the solution; Aliquot into microcentrifuge tubes and store at −20°C; Avoid repeated freeze/thaw cycles

Applications:
ChIP Assay, Immunoprecipitation, Western Blot

Conjugate:
Unconjugated

Gene Accession No.:
P62805

Host Species:
Rabbit

Purification Method:
Unpurified

Regulatory Status:
RUO

Primary or Secondary:
Primary

Test Specificity:
Recognizes acetyl-Histone H4 (Lys8).

Target Species:
Human, S. cerevisiae, Yeast

Form:
Serum

Img

MilliporeSigma™

17-101-01MI

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Antigen:
Acetyl-Histone H4 (Lys16)

Classification:
Polyclonal

Formulation:
Anti-Acetyl-Histone H4 (Lys16) (rabbit polyclonal). One vial containing 50μL of affinity purified rabbit polyclonal serum in buffer containing 0.1 M Tris-Glycine (pH 7.4) 150mM NaCl with 0.05% sodium azide. Normal Rabbit IgG. One vial containing 125μg of rabbit IgG in 125μL of storage buffer containing 0.05% sodium azide. ChIP Primers, RPL10 promoter. One vial containing 75μL of 5μM of each primer specific for the promoter region of human RPL10. FOR: ACC CGT CTT CGA CAG GAC T; REV: GGA ACG GAA GAC GAG AAC AG

Gene Symbols:
HIST1H4A, H4/J, HIST1H4F, HIST1H4L, H4FN, H4FA, H4FH, HIST1H4H, H4F2, H4FM, H4/A, H4FK, H4FG, HIST2H4, H4FB, H4/K, HIST1H4I, H4/N, HIST1H4B, H4FE, H4/M, H4/a, HIST1H4E, HIST4H4, HIST2H4A, H4FC, H4FI, H4/H, HIST1H4C, H4/G, HIST1H4K, H4FJ, H4FD, H4/I, H4/B, H4/D, H4/C, HIST1H4J, HIST1H4D, H4/E

Immunogen:
KLH-conjugated Histone H4 corresponding to the N-terminus region at and around acetylated Lys16.

Quantity:
25 Assays

Research Discipline:
Epigenetics and Nuclear Function

Gene ID (Entrez):
NP_778224.1

Includes:
This ChIPAb+ Acetyl-Histone H4 (Lys16) -ChIP Validated Antibody and Primer Set conveniently includes the antibody and the specific control PCR primers.

Content And Storage:
-20°C for up to 12 months from date of receipt; Upon first thaw and prior to removing the cap centrifuge the vial and gently mix the solution; Aliquot into microcentrifuge tubes and store at −20°C; Avoid repeated freeze/thaw cycles

Applications:
ChIP Assay, Immunoprecipitation, Western Blot

Conjugate:
Unconjugated

Gene Accession No.:
P62805

Host Species:
Rabbit

Purification Method:
Affinity Purified

Regulatory Status:
RUO

Primary or Secondary:
Primary

Test Specificity:
This antibody detects histone H4 acetylated at Lys16.

Target Species:
Human, Vertebrates

Form:
Purified

Img

MilliporeSigma™

17-101-09MI

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Antigen:
TCF-4α

Classification:
Monoclonal

Formulation:
Anti-TCF-4 (mouse monoclonal). One vial containing 50μg of purified mouse monoclonal IgG2a in 50μL of buffer containing 0.1 M Tris-glycine, pH 7.4, 0.15 M NaCl, 0.05% sodium azide and 30% glycerol. Normal Mouse IgG. Two vials containing 25μg of purified IgG in 25μL of storage buffer containing 0.1% sodium azide. ChIP Primers, SP5 promote. One vial containing 75μL of 5μM of each primer specific for human SP5 promotor. FOR: GGG TCT CCA GGC GGC AAG; REV: AGC GAA AGC AAA TCC TTT GAA TCC

Gene Symbols:
TCF4, TCF-4, TCF7L2

Immunogen:
Amino acids 31-331 of human TCF-4.

Quantity:
25 Assays

Research Discipline:
Epigenetics and Nuclear Function

Gene ID (Entrez):
CAB97213.1

Includes:
This ChIPAb+ TCF-4 -ChIP Validated Antibody and Primer Set conveniently includes the antibody and the specific control PCR primers.

Content And Storage:
-20°C for one year from date of shipment; Upon first thaw and prior to removing the cap centrifuge the vial and gently mix the solution; Aliquot into microcentrifuge tubes and store at −20°C; Avoid repeated freeze/thaw cycles

Applications:
ChIP Assay, Dot Blot, Western Blot

Conjugate:
Unconjugated

Gene Accession No.:
Q9NQB0

Host Species:
Mouse

Purification Method:
Protein G chromatography

Regulatory Status:
RUO

Primary or Secondary:
Primary

Test Specificity:
This antibody recognizes TCF-4

Target Species:
Human, Mouse

Form:
Purified

Img

MilliporeSigma™

17-101-11MI

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Antigen:
Acetyl-Histone H3 (Lys18)

Classification:
Polyclonal

Formulation:
Anti-Acetyl-Histone H3 (Lys18) (rabbit polyclonal). One vial of 50μL of rabbit serum containing 0.05% sodium azide and 30% glycerol. Normal Rabbit Serum. One vial of 100μL of antiserum containing 0.05% sodium azide. Control Primers, human GAPDH promoter. One vial containing 75μL of 5μM each of primer specific for human GAPDH promoter. FOR: TAC TAG CGG TTT TAC GGG CG; REV: TCG AAC AGG AGG AGC AGA GAG CGA

Gene Symbols:
HIST3H3, H3FT, MGC126886, H3t, MGC126888, H3T, H3/g, H3.4, H3/t

Immunogen:
KLH-conjugated, synthetic peptide (GKAPRAcKQLASK-C) corresponding to amino acids 13-23 of yeast Histone H3 acetylated on lysine 18with a C-terminal cysteine added for conjugation purposes

Quantity:
25 Assays

Research Discipline:
Epigenetics and Nuclear Function

Gene ID (Entrez):
NM_003493

Includes:
This ChIPAb+ Acetyl-Histone H3 (Lys18) -ChIP Validated Antibody and Primer Set conveniently includes the antibody and the specific control PCR primers.

Content And Storage:
-20°C for up to 12 months from date of receipt; Upon first thaw and prior to removing the cap centrifuge the vial and gently mix the solution; Aliquot into microcentrifuge tubes and store at −20°C; Avoid repeated freeze/thaw cycles

Applications:
ChIP Assay, ChIP sequencing (ChIP-seq), Dot Blot, Western Blot

Conjugate:
Unconjugated

Gene Accession No.:
Q16695

Host Species:
Rabbit

Purification Method:
__

Regulatory Status:
RUO

Primary or Secondary:
Primary

Test Specificity:
Recognizes Histone H3 acetylated on lysine 18.

Target Species:
Human, S. cerevisiae, Vertebrates, Yeast

Form:
Serum