7090816

Mouse IgG2b kappa Isotype Control (eBMG2b), PerCP-eFluor™ 710, eBioscience™, Invitrogen™

Mouse Isotype Control

Manufacturer: Fischer Scientific

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Antigen

Mouse IgG2b kappa

Concentration

0.2 mg/mL

Conjugate

PerCP-eFluor 710

Host Species

Mouse

Target Species

Not Applicable

Gene Alias

IgG2; Immunoglobulin G; Immunoglobulin G2; ImmunoglobulinG; ImmunoglobulinG2

Primary or Secondary

Isotype Control (Primary)

Content And Storage

4° C, store in dark, DO NOT FREEZE!

Clone

eBMG2b

Applications

Control, Flow Cytometry

Form

Liquid

Regulatory Status

RUO

Formulation

PBS with 0.09% sodium azide; pH 7.2

Isotype

IgG2b κ

Purification Method

Affinity chromatography

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Description

  • Description: The monoclonal mouse IgG2b, kappa is useful as an isotype control immunoglobulin
  • Applications Reported: Mouse IgG2b Isotype Control PerCP-eFluor® 710 has been reported for use in flow cytometric analysis
  • Applications Tested: Mouse IgG2b K Isotype Control PerCP-eFluor® 710 has been tested by flow ctometric analysis of normal human peripheral blood cells
  • Use isotype control at the same concentration as experimental antibody
  • PerCP-eFluor® 710 can be used in place of PE-Cy5, PE-Cy5.5 or PerCP-Cy5.5
  • PerCP-eFluor® 710 emits at 710 nm and is excited with the blue laser (488 nm)
  • Please make sure that your instrument is capable of detecting this fluorochrome
  • For a filter configuration, we recommend using the 685 LP dichroic mirror and 710/40 band pass filter, however the 695/40 band pass filter is an acceptable alternative
  • Our testing indicates that PerCP-eFluor® 710 conjugated antibodies are stable when stained samples are exposed to freshly prepared 2% formaldehyde overnight at 4°C, but please evaluate for alternative fixation protocols
  • Excitation: 488 nm; Emission: 710 nm; Laser: Blue Laser
  • Filtration: 0.2 μm post-manufacturing filtered
  • Mouse IgG2b is a subtype of immunoglobulin G antibody that is produced by B cells in response to an antigen
  • It is the third most abundant IgG subtype in the serum and plays a crucial role in the adaptive immune response
  • Mouse IgG2b is composed of two heavy chains and two light chains, and it is involved in opsonization, complement activation, and antibody-dependent cell-mediated cytotoxicity
  • It is also involved in the regulation of immune responses, including the activation of T cells and the inhibition of B cell activation
  • Mouse IgG2b is widely used in research and diagnostic applications, including ELISA, Western blotting, and immunohistochemistry
  • It is also used in therapeutic applications, such as immunotherapy and vaccine development.

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Mouse Isotype Control...


Antigen:
Mouse IgG2b kappa

Concentration:
0.2 mg/mL

Conjugate:
PerCP-eFluor 710

Host Species:
Mouse

Target Species:
Not Applicable

Gene Alias:
IgG2; Immunoglobulin G; Immunoglobulin G2; ImmunoglobulinG; ImmunoglobulinG2

Primary or Secondary:
Isotype Control (Primary)

Content And Storage:
4° C, store in dark, DO NOT FREEZE!

Clone:
eBMG2b

Applications:
Control, Flow Cytometry

Form:
Liquid

Regulatory Status:
RUO

Formulation:
PBS with 0.09% sodium azide; pH 7.2

Isotype:
IgG2b κ

Purification Method:
Affinity chromatography

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Fischer Scientific

7090819

Mouse Monoclonal Antibody...


Antigen:
EOMES

Concentration:
5 μL/Test

Conjugate:
PerCP-eFluor 710

Host Species:
Mouse

Target Species:
Human, Porcine

Gene Alias:
C77258; EOMES; eomesodermin; eomesodermin homolog; eomesodermin homolog (Xenopus laevis); T-box brain protein 2; T-box transcription factor; TBR2; TBR-2; T-brain-2

Primary or Secondary:
Primary

Content And Storage:
4° C, store in dark, DO NOT FREEZE!

Clone:
WD1928

Applications:
Flow Cytometry

Form:
Liquid

Regulatory Status:
RUO

Formulation:
PBS with 0.2% BSA and 0.09% sodium azide; pH 7.2

Isotype:
IgG1 κ

Purification Method:
Affinity chromatography

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Armenian Hamster Isotype Control...


Antigen:
Armenian Hamster IgG

Concentration:
0.2 mg/mL

Conjugate:
PerCP-eFluor 710

Host Species:
Armenian Hamster

Target Species:
Not Applicable

Gene Alias:
IgG; Immunoglobulin G; ImmunoglobulinG

Primary or Secondary:
Isotype Control (Primary)

Content And Storage:
4° C, store in dark, DO NOT FREEZE!

Clone:
eBio299Arm

Applications:
Control, Flow Cytometry

Form:
Liquid

Regulatory Status:
RUO

Formulation:
PBS with 0.09% sodium azide; pH 7.2

Isotype:
IgG

Purification Method:
Affinity chromatography

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Rat Monoclonal Antibody...


Antigen:
MHC Class II (I-A/I-E)

Concentration:
0.2 mg/mL

Conjugate:
PerCP-eFluor 710

Host Species:
Rat

Target Species:
Mouse

Gene Alias:
Abeta; AI323765; AI845868; cell surface glycoprotein; E-alpha-f; H-2 class II histocompatibility antigen, A beta chain; H-2 class II histocompatibility antigen, E-D alpha chain; H-2 class II histocompatibility antigen, E-K alpha chain; H-2 class II histocompatibility antigen, E-U alpha chain; H-2 class II histocompatibility antigen, I-E beta chain; H-2Ab; H2-Ab; H2-Ab1; H-2Ea; H2-Ea; H2-Ea-ps; H2Eb; H-2Eb; H2-Eb1; H2-iabeta; H2-IE-alpha; histocompatibility 2, class II antigen A, beta 1; histocompatibility 2, class II antigen E alpha; histocompatibility 2, class II antigen E alpha, pseudogene; histocompatibility 2, class II antigen E beta; Ia2; Ia-2; Ia3; Ia-3; Ia4; Ia-4; IAb; I-Ab; I-Abeta; I-Ad; I-Aq; I-E alpha MHC class II; I-E beta MHC class II; I-Ealpha; I-Ed; I-Ek; major histocompatibility complex class II beta chain; major histocompatibility complex H-2E beta chain; MHC class 2; MHC class II antigen A beta; MHC class II antigen E alpha; MHC class II H2-IA-beta-psi; MHC class II IE-b; MHC class II protein; MHC H2-IE-beta cell surface glycoprotein; response to metastatic cancers 1; Rmcs1

Primary or Secondary:
Primary

Content And Storage:
4° C, store in dark, DO NOT FREEZE!

Clone:
M5/114.15.2

Applications:
Flow Cytometry

Form:
Liquid

Regulatory Status:
RUO

Formulation:
PBS with 0.09% sodium azide; pH 7.2

Isotype:
IgG2b κ

Purification Method:
Affinity chromatography