7090815

Mouse IgG2a kappa Isotype Control (eBM2a), PerCP-eFluor™ 710, eBioscience™, Invitrogen™

Mouse Isotype Control

Manufacturer: Fischer Scientific

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Antigen

Mouse IgG2a kappa

Concentration

0.2 mg/mL

Conjugate

PerCP-eFluor 710

Host Species

Mouse

Target Species

Not Applicable

Gene Alias

IgG2; Immunoglobulin G; Immunoglobulin G2; ImmunoglobulinG; ImmunoglobulinG2

Primary or Secondary

Isotype Control (Primary)

Content And Storage

4° C, store in dark, DO NOT FREEZE!

Clone

eBM2a

Applications

Control, Flow Cytometry

Form

Liquid

Regulatory Status

RUO

Formulation

PBS with 0.09% sodium azide; pH 7.2

Isotype

IgG2a κ

Purification Method

Affinity chromatography

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Description

  • Description: This is a monoclonal mouse IgG2a, kappa antibody
  • It is used as an isotype control for mouse IgG2a antibodies
  • Applications Reported: PerCP-eFluor® 710 Mouse IgG2a, K Isotype Control has been reported for use in flow cytometric analysis
  • Applications Tested: PerCP-eFluor® 710 Mouse IgG2a Isotype Control has been tested by flow cytometric analysis of normal human peripheral blood cells and mouse spleen cells.Use at the same concentration as experimental antibody
  • PerCP-eFluor® 710 can be used in place of PE-Cy5, PE-Cy5.5 or PerCP-Cy5.5
  • PerCP-eFluor® 710 emits at 710 nm and is excited with the blue laser (488 nm)
  • Please make sure that your instrument is capable of detecting this fluorochrome
  • For a filter configuration, we recommend using the 685 LP dichroic mirror and 710/40 band pass filter, however the 695/40 band pass filter is an acceptable alternative
  • Our testing indicates that PerCP-eFluor® 710 conjugated antibodies are stable when stained samples are exposed to freshly prepared 2% formaldehyde overnight at 4°C, but please evaluate for alternative fixation protocols
  • Excitation: 488 nm; Emission: 710 nm; Laser: Blue Laser
  • Filtration: 0.2 μm post-manufacturing filtered
  • Mouse IgG2a is a subtype of immunoglobulin G antibody that is produced by B cells in response to an antigen
  • It is the second most abundant IgG subtype in the serum and plays a crucial role in the adaptive immune response
  • Mouse IgG2a is composed of two heavy chains and two light chains, and it is involved in opsonization, complement activation, and antibody-dependent cell-mediated cytotoxicity
  • It is also involved in the regulation of immune responses, including the activation of T cells and the inhibition of B cell activation
  • Mouse IgG2a is widely used in research and diagnostic applications, including ELISA, Western blotting, and immunohistochemistry
  • It is also used in therapeutic applications, such as immunotherapy and vaccine development.

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7090815

Mouse Isotype Control...


Antigen:
Mouse IgG2a kappa

Concentration:
0.2 mg/mL

Conjugate:
PerCP-eFluor 710

Host Species:
Mouse

Target Species:
Not Applicable

Gene Alias:
IgG2; Immunoglobulin G; Immunoglobulin G2; ImmunoglobulinG; ImmunoglobulinG2

Primary or Secondary:
Isotype Control (Primary)

Content And Storage:
4° C, store in dark, DO NOT FREEZE!

Clone:
eBM2a

Applications:
Control, Flow Cytometry

Form:
Liquid

Regulatory Status:
RUO

Formulation:
PBS with 0.09% sodium azide; pH 7.2

Isotype:
IgG2a κ

Purification Method:
Affinity chromatography

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Mouse Isotype Control...


Antigen:
Mouse IgG2b kappa

Concentration:
0.2 mg/mL

Conjugate:
PerCP-eFluor 710

Host Species:
Mouse

Target Species:
Not Applicable

Gene Alias:
IgG2; Immunoglobulin G; Immunoglobulin G2; ImmunoglobulinG; ImmunoglobulinG2

Primary or Secondary:
Isotype Control (Primary)

Content And Storage:
4° C, store in dark, DO NOT FREEZE!

Clone:
eBMG2b

Applications:
Control, Flow Cytometry

Form:
Liquid

Regulatory Status:
RUO

Formulation:
PBS with 0.09% sodium azide; pH 7.2

Isotype:
IgG2b κ

Purification Method:
Affinity chromatography

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Antigen:
EOMES

Concentration:
5 μL/Test

Conjugate:
PerCP-eFluor 710

Host Species:
Mouse

Target Species:
Human, Porcine

Gene Alias:
C77258; EOMES; eomesodermin; eomesodermin homolog; eomesodermin homolog (Xenopus laevis); T-box brain protein 2; T-box transcription factor; TBR2; TBR-2; T-brain-2

Primary or Secondary:
Primary

Content And Storage:
4° C, store in dark, DO NOT FREEZE!

Clone:
WD1928

Applications:
Flow Cytometry

Form:
Liquid

Regulatory Status:
RUO

Formulation:
PBS with 0.2% BSA and 0.09% sodium azide; pH 7.2

Isotype:
IgG1 κ

Purification Method:
Affinity chromatography

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Antigen:
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Concentration:
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Conjugate:
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Host Species:
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Target Species:
Not Applicable

Gene Alias:
IgG; Immunoglobulin G; ImmunoglobulinG

Primary or Secondary:
Isotype Control (Primary)

Content And Storage:
4° C, store in dark, DO NOT FREEZE!

Clone:
eBio299Arm

Applications:
Control, Flow Cytometry

Form:
Liquid

Regulatory Status:
RUO

Formulation:
PBS with 0.09% sodium azide; pH 7.2

Isotype:
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Purification Method:
Affinity chromatography