7091026
Rat IgG2b kappa Isotype Control (eB149/10H5), APC-eFluor™ 780, eBioscience™, Invitrogen™
Rat Isotype Control
Manufacturer: Fischer Scientific
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Antigen
Rat IgG2b kappa
Concentration
0.2 mg/mL
Conjugate
APC-eFluor 780
Host Species
Rat
Target Species
Not Applicable
Gene Alias
IgG2; Immunoglobulin G; Immunoglobulin G2; ImmunoglobulinG; ImmunoglobulinG2
Primary or Secondary
Isotype Control (Primary)
Content And Storage
4° C, store in dark, DO NOT FREEZE!
Clone
eB149/10H5
Applications
Control, Flow Cytometry
Form
Liquid
Regulatory Status
RUO
Formulation
PBS with 0.09% sodium azide; pH 7.2
Isotype
IgG2b κ
Purification Method
Affinity chromatography
Related Products
Description
- Description: The rat IgG2b, kappa monoclonal antibody is useful as an isotype control immunoglobulin
- Applications Reported: APC-eFluor™ 780 Rat IgG2b Isotype Control (APC-Alexa Fluor™ 750 replacement) has been reported for use in flow cytometric analysis
- Applications Tested: Rat IgG2b K Isotype Control APC-eFluor™ 780 has been tested by flow cytometric analysis of mouse splenocytes
- Use isotype control at the same concentration as experimental antibody
- APC-eFluor 780 emits at 780 nm and is excited with the Red laser (633 nm)
- Please make sure that your instrument is capable of detecting this fluorochome
- Light sensitivity: This tandem is sensitive to photo-induced oxidation
- Please protect this vial and stained samples from light
- Fixation: Samples can be stored in IC Fixation Buffer (cat
- 00-8222) (100 μL cell sample + 100 μL IC Fixation Buffer) or 1-step Fix/Lyse Solution (cat
- 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation
- Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically
- Excitation: 633-647 nm; Emission: 780 nm; Laser: Red Laser
- Filtration: 0.2 μm post-manufacturing filtered
- Rat IgG2b is a protein-coding gene that encodes for the immunoglobulin G2b (IgG2b) subclass of rat antibodies
- IgG2b is a major subclass of rat immunoglobulins that plays a crucial role in the immune response by neutralizing pathogens and promoting opsonization
- Rat IgG2b is commonly used in research applications, such as in immunohistochemistry, flow cytometry, and Western blotting.
Compare Similar Items
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Antigen: Rat IgG2b kappa
Concentration: 0.2 mg/mL
Conjugate: APC-eFluor 780
Host Species: Rat
Target Species: Not Applicable
Gene Alias: IgG2; Immunoglobulin G; Immunoglobulin G2; ImmunoglobulinG; ImmunoglobulinG2
Primary or Secondary: Isotype Control (Primary)
Content And Storage: 4° C, store in dark, DO NOT FREEZE!
Clone: eB149/10H5
Applications: Control, Flow Cytometry
Form: Liquid
Regulatory Status: RUO
Formulation: PBS with 0.09% sodium azide; pH 7.2
Isotype: IgG2b κ
Purification Method: Affinity chromatography
Antigen:
Rat IgG2b kappa
Concentration:
0.2 mg/mL
Conjugate:
APC-eFluor 780
Host Species:
Rat
Target Species:
Not Applicable
Gene Alias:
IgG2; Immunoglobulin G; Immunoglobulin G2; ImmunoglobulinG; ImmunoglobulinG2
Primary or Secondary:
Isotype Control (Primary)
Content And Storage:
4° C, store in dark, DO NOT FREEZE!
Clone:
eB149/10H5
Applications:
Control, Flow Cytometry
Form:
Liquid
Regulatory Status:
RUO
Formulation:
PBS with 0.09% sodium azide; pH 7.2
Isotype:
IgG2b κ
Purification Method:
Affinity chromatography
Antigen: Mouse IgG1 kappa
Concentration: 0.2 mg/mL
Conjugate: APC-eFluor 780
Host Species: Mouse
Target Species: Not Applicable
Gene Alias: IgG1; Igh-4; Ighg1; Immunoglobulin G; Immunoglobulin G1; immunoglobulin heavy constant gamma 1 (G1m marker); ImmunoglobulinG; ImmunoglobulinG1; VH7183
Primary or Secondary: Isotype Control (Primary)
Content And Storage: 4° C, store in dark, DO NOT FREEZE!
Clone: P3.6.2.8.1
Applications: Control, Flow Cytometry
Form: Liquid
Regulatory Status: RUO
Formulation: PBS with 0.09% sodium azide; pH 7.2
Isotype: IgG1 κ
Purification Method: Affinity chromatography
Antigen:
Mouse IgG1 kappa
Concentration:
0.2 mg/mL
Conjugate:
APC-eFluor 780
Host Species:
Mouse
Target Species:
Not Applicable
Gene Alias:
IgG1; Igh-4; Ighg1; Immunoglobulin G; Immunoglobulin G1; immunoglobulin heavy constant gamma 1 (G1m marker); ImmunoglobulinG; ImmunoglobulinG1; VH7183
Primary or Secondary:
Isotype Control (Primary)
Content And Storage:
4° C, store in dark, DO NOT FREEZE!
Clone:
P3.6.2.8.1
Applications:
Control, Flow Cytometry
Form:
Liquid
Regulatory Status:
RUO
Formulation:
PBS with 0.09% sodium azide; pH 7.2
Isotype:
IgG1 κ
Purification Method:
Affinity chromatography
Antigen: EOMES
Concentration: 5 μL/Test
Conjugate: APC-eFluor 780
Host Species: Mouse
Target Species: Human, Porcine
Gene Alias: C77258; EOMES; eomesodermin; eomesodermin homolog; eomesodermin homolog (Xenopus laevis); T-box brain protein 2; T-box transcription factor; TBR2; TBR-2; T-brain-2
Primary or Secondary: Primary
Content And Storage: 4° C, store in dark, DO NOT FREEZE!
Clone: WD1928
Applications: Flow Cytometry
Form: Liquid
Regulatory Status: RUO
Formulation: PBS with 0.2% BSA and 0.09% sodium azide; pH 7.2
Isotype: IgG1 κ
Purification Method: Affinity chromatography
Antigen:
EOMES
Concentration:
5 μL/Test
Conjugate:
APC-eFluor 780
Host Species:
Mouse
Target Species:
Human, Porcine
Gene Alias:
C77258; EOMES; eomesodermin; eomesodermin homolog; eomesodermin homolog (Xenopus laevis); T-box brain protein 2; T-box transcription factor; TBR2; TBR-2; T-brain-2
Primary or Secondary:
Primary
Content And Storage:
4° C, store in dark, DO NOT FREEZE!
Clone:
WD1928
Applications:
Flow Cytometry
Form:
Liquid
Regulatory Status:
RUO
Formulation:
PBS with 0.2% BSA and 0.09% sodium azide; pH 7.2
Isotype:
IgG1 κ
Purification Method:
Affinity chromatography
Antigen: MHC Class II (I-A/I-E)
Concentration: 0.2 mg/mL
Conjugate: APC-eFluor 780
Host Species: Rat
Target Species: Mouse
Gene Alias: Abeta; AI323765; AI845868; cell surface glycoprotein; E-alpha-f; H-2 class II histocompatibility antigen, A beta chain; H-2 class II histocompatibility antigen, E-D alpha chain; H-2 class II histocompatibility antigen, E-K alpha chain; H-2 class II histocompatibility antigen, E-U alpha chain; H-2 class II histocompatibility antigen, I-E beta chain; H-2Ab; H2-Ab; H2-Ab1; H-2Ea; H2-Ea; H2-Ea-ps; H2Eb; H-2Eb; H2-Eb1; H2-iabeta; H2-IE-alpha; histocompatibility 2, class II antigen A, beta 1; histocompatibility 2, class II antigen E alpha; histocompatibility 2, class II antigen E alpha, pseudogene; histocompatibility 2, class II antigen E beta; Ia2; Ia-2; Ia3; Ia-3; Ia4; Ia-4; IAb; I-Ab; I-Abeta; I-Ad; I-Aq; I-E alpha MHC class II; I-E beta MHC class II; I-Ealpha; I-Ed; I-Ek; major histocompatibility complex class II beta chain; major histocompatibility complex H-2E beta chain; MHC class 2; MHC class II antigen A beta; MHC class II antigen E alpha; MHC class II H2-IA-beta-psi; MHC class II IE-b; MHC class II protein; MHC H2-IE-beta cell surface glycoprotein; response to metastatic cancers 1; Rmcs1
Primary or Secondary: Primary
Content And Storage: 4° C, store in dark, DO NOT FREEZE!
Clone: M5/114.15.2
Applications: Flow Cytometry
Form: Liquid
Regulatory Status: RUO
Formulation: PBS with 0.09% sodium azide; pH 7.2
Isotype: IgG2b κ
Purification Method: Affinity chromatography
Antigen:
MHC Class II (I-A/I-E)
Concentration:
0.2 mg/mL
Conjugate:
APC-eFluor 780
Host Species:
Rat
Target Species:
Mouse
Gene Alias:
Abeta; AI323765; AI845868; cell surface glycoprotein; E-alpha-f; H-2 class II histocompatibility antigen, A beta chain; H-2 class II histocompatibility antigen, E-D alpha chain; H-2 class II histocompatibility antigen, E-K alpha chain; H-2 class II histocompatibility antigen, E-U alpha chain; H-2 class II histocompatibility antigen, I-E beta chain; H-2Ab; H2-Ab; H2-Ab1; H-2Ea; H2-Ea; H2-Ea-ps; H2Eb; H-2Eb; H2-Eb1; H2-iabeta; H2-IE-alpha; histocompatibility 2, class II antigen A, beta 1; histocompatibility 2, class II antigen E alpha; histocompatibility 2, class II antigen E alpha, pseudogene; histocompatibility 2, class II antigen E beta; Ia2; Ia-2; Ia3; Ia-3; Ia4; Ia-4; IAb; I-Ab; I-Abeta; I-Ad; I-Aq; I-E alpha MHC class II; I-E beta MHC class II; I-Ealpha; I-Ed; I-Ek; major histocompatibility complex class II beta chain; major histocompatibility complex H-2E beta chain; MHC class 2; MHC class II antigen A beta; MHC class II antigen E alpha; MHC class II H2-IA-beta-psi; MHC class II IE-b; MHC class II protein; MHC H2-IE-beta cell surface glycoprotein; response to metastatic cancers 1; Rmcs1
Primary or Secondary:
Primary
Content And Storage:
4° C, store in dark, DO NOT FREEZE!
Clone:
M5/114.15.2
Applications:
Flow Cytometry
Form:
Liquid
Regulatory Status:
RUO
Formulation:
PBS with 0.09% sodium azide; pH 7.2
Isotype:
IgG2b κ
Purification Method:
Affinity chromatography