Classification:
Polyclonal
Formulation:
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Immunogen:
This GFP antibody was developed against a fusion protein corresponding to the full length GFP (246aa) derived from the jellyfish Aequorea victoria.
Test Specificity:
GFP antibody was prepared from monospecific antiserum by immunoaffinity chromatography using Green Fluorescent Protein (Aequorea victoria) coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum and purified and partially purified Green Fluorescent Protein (Aequorea victoria).
Content And Storage:
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Applications:
Western Blot, ELISA, Immunocytochemistry, Immunofluorescence
Dilution:
Western Blot 1:100 - 1:2000, ELISA 1:100-1:2000, Immunocytochemistry/Immunofluorescence 1:500-1:2500, Fluorophore-linked immunosorbent assay 1:100 - 1:2000
Target Species:
Non-species specific