NBP231381
anti-Rat IgG2a, kappa, Isotype Control, Novus Biologicals™
Manufacturer: Novus Biologicals
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Antigen
IgG2a Kappa
Concentration
1mg/mL
Dilution
Western Blot 1:100 - 1:2000, Flow Cytometry 1:1000 - 1:5000, ELISA 1:100 - 1:2000, Immunocytochemistry/Immunofluorescence 1:10 - 1:500, Fluorophore-linked immunosorbent assay 1:100 - 1:2000
Host Species
Rat
Quantity
0.5 mg
Primary or Secondary
Isotype Control (Primary)
Target Species
Rat
Form
Purified
Applications
Western Blot, Flow Cytometry, ELISA, Immunocytochemistry, Immunofluorescence
Conjugate
Unconjugated
Formulation
0.02 M Potassium Phosphate, 0.5 M Sodium Chloride, pH 7.2. Stabilizer: None. with 0.01% Sodium Azide
Purification Method
Protein G purified
Regulatory Status
RUO
Test Specificity
In an Ouchterlony double diffusion assay the material is non-reactive with antisera to rat IgG1a, IgG1b, IgG3, IgM, and IgA. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rat IgG and anti-Rat serum.
Content And Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Isotype
IgG2a κ
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Antigen: IgG2a Kappa
Concentration: 1mg/mL
Dilution: Western Blot 1:100 - 1:2000, Flow Cytometry 1:1000 - 1:5000, ELISA 1:100 - 1:2000, Immunocytochemistry/Immunofluorescence 1:10 - 1:500, Fluorophore-linked immunosorbent assay 1:100 - 1:2000
Host Species: Rat
Quantity: 0.5 mg
Primary or Secondary: Isotype Control (Primary)
Target Species: Rat
Form: Purified
Applications: Western Blot, Flow Cytometry, ELISA, Immunocytochemistry, Immunofluorescence
Conjugate: Unconjugated
Formulation: 0.02 M Potassium Phosphate, 0.5 M Sodium Chloride, pH 7.2. Stabilizer: None. with 0.01% Sodium Azide
Purification Method: Protein G purified
Regulatory Status: RUO
Test Specificity: In an Ouchterlony double diffusion assay the material is non-reactive with antisera to rat IgG1a, IgG1b, IgG3, IgM, and IgA. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rat IgG and anti-Rat serum.
Content And Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Isotype: IgG2a κ
Antigen:
IgG2a Kappa
Concentration:
1mg/mL
Dilution:
Western Blot 1:100 - 1:2000, Flow Cytometry 1:1000 - 1:5000, ELISA 1:100 - 1:2000, Immunocytochemistry/Immunofluorescence 1:10 - 1:500, Fluorophore-linked immunosorbent assay 1:100 - 1:2000
Host Species:
Rat
Quantity:
0.5 mg
Primary or Secondary:
Isotype Control (Primary)
Target Species:
Rat
Form:
Purified
Applications:
Western Blot, Flow Cytometry, ELISA, Immunocytochemistry, Immunofluorescence
Conjugate:
Unconjugated
Formulation:
0.02 M Potassium Phosphate, 0.5 M Sodium Chloride, pH 7.2. Stabilizer: None. with 0.01% Sodium Azide
Purification Method:
Protein G purified
Regulatory Status:
RUO
Test Specificity:
In an Ouchterlony double diffusion assay the material is non-reactive with antisera to rat IgG1a, IgG1b, IgG3, IgM, and IgA. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rat IgG and anti-Rat serum.
Content And Storage:
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Isotype:
IgG2a κ
Antigen: IgG2a Lambda
Concentration: 1.1mg/mL
Dilution: Western Blot 1:100 - 1:2000, Flow Cytometry 1:1000 - 1:5000, ELISA 1:100 - 1:2000, Immunocytochemistry/Immunofluorescence 1:10 - 1:500, Fluorophore-linked immunosorbent assay 1:100 - 1:2000
Host Species: Rat
Quantity: 0.5 mg
Primary or Secondary: Isotype Control (Primary)
Target Species: Rat
Form: Purified
Applications: Western Blot, Flow Cytometry, ELISA, Immunocytochemistry, Immunofluorescence
Conjugate: Unconjugated
Formulation: 0.02 M Potassium Phosphate, 0.5 M Sodium Chloride, pH 7.2. Stabilizer: None. with 0.01% Sodium Azide
Purification Method: Protein G purified
Regulatory Status: RUO
Test Specificity: Isotyping assay resulted non-reactive with antisera to Rat IgG1a, IgG1b, IgG3, IgM, and IgA
Content And Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Isotype: IgG2a
Antigen:
IgG2a Lambda
Concentration:
1.1mg/mL
Dilution:
Western Blot 1:100 - 1:2000, Flow Cytometry 1:1000 - 1:5000, ELISA 1:100 - 1:2000, Immunocytochemistry/Immunofluorescence 1:10 - 1:500, Fluorophore-linked immunosorbent assay 1:100 - 1:2000
Host Species:
Rat
Quantity:
0.5 mg
Primary or Secondary:
Isotype Control (Primary)
Target Species:
Rat
Form:
Purified
Applications:
Western Blot, Flow Cytometry, ELISA, Immunocytochemistry, Immunofluorescence
Conjugate:
Unconjugated
Formulation:
0.02 M Potassium Phosphate, 0.5 M Sodium Chloride, pH 7.2. Stabilizer: None. with 0.01% Sodium Azide
Purification Method:
Protein G purified
Regulatory Status:
RUO
Test Specificity:
Isotyping assay resulted non-reactive with antisera to Rat IgG1a, IgG1b, IgG3, IgM, and IgA
Content And Storage:
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Isotype:
IgG2a
Antigen: TAG-72
Concentration: 0.2mg/mL
Dilution: Western Blot 1:200-1:400, Flow Cytometry 0.5ug/million cells, ELISA 1-5ug/ml for coating, Immunocytochemistry/Immunofluorescence 1:100-1:200, Immunoprecipitation 1-2ug/500ug protein lysate, Immunohistochemistry-Paraffin 1:200-1:400, Immunohistochemistry-Frozen 1:200-1:400
Host Species: Mouse
Quantity: 0.1 mg
Primary or Secondary: Primary
Target Species: Human, Rat, Bovine, Canine
Form: Purified
Applications: Western Blot, Flow Cytometry, ELISA, Immunocytochemistry, Immunofluorescence, Immunoprecipitation, Immunohistochemistry (Paraffin)
Conjugate: Unconjugated
Formulation: PBS with 0.05% BSA. with 0.05% Sodium Azide
Purification Method: Protein A purified
Regulatory Status: RUO
Test Specificity: Recognizes an oncofetal antigen of 220kDa, identified as a tumor-associated glycoprotein (TAG-72) with properties of a mucin. This MAb defines the mucin-carried sialylated-Tn epitope. TAG-72 is usually expressed by adenocarcinomas, but is negative in mesotheliomas. Studies have reported that this antibody has 80% sensitivity and 93% specificity for pulmonary adenocarcinoma. Therefore, TAG-72 is a useful marker to distinguish between mesothelioma and adenocarcinoma. However, false positive reactions can occur so results must be interpreted with the utmost caution. This antibody may be useful in the differentiation of non-small cell carcinomas from small cell carcinomas of the lung. The combined use of anti-TAG-72 and anti-GCDFP-15 is valuable in the diagnosis of apocrine carcinoma.
Content And Storage: Store at 4C.
Isotype: IgG1 κ
Antigen:
TAG-72
Concentration:
0.2mg/mL
Dilution:
Western Blot 1:200-1:400, Flow Cytometry 0.5ug/million cells, ELISA 1-5ug/ml for coating, Immunocytochemistry/Immunofluorescence 1:100-1:200, Immunoprecipitation 1-2ug/500ug protein lysate, Immunohistochemistry-Paraffin 1:200-1:400, Immunohistochemistry-Frozen 1:200-1:400
Host Species:
Mouse
Quantity:
0.1 mg
Primary or Secondary:
Primary
Target Species:
Human, Rat, Bovine, Canine
Form:
Purified
Applications:
Western Blot, Flow Cytometry, ELISA, Immunocytochemistry, Immunofluorescence, Immunoprecipitation, Immunohistochemistry (Paraffin)
Conjugate:
Unconjugated
Formulation:
PBS with 0.05% BSA. with 0.05% Sodium Azide
Purification Method:
Protein A purified
Regulatory Status:
RUO
Test Specificity:
Recognizes an oncofetal antigen of 220kDa, identified as a tumor-associated glycoprotein (TAG-72) with properties of a mucin. This MAb defines the mucin-carried sialylated-Tn epitope. TAG-72 is usually expressed by adenocarcinomas, but is negative in mesotheliomas. Studies have reported that this antibody has 80% sensitivity and 93% specificity for pulmonary adenocarcinoma. Therefore, TAG-72 is a useful marker to distinguish between mesothelioma and adenocarcinoma. However, false positive reactions can occur so results must be interpreted with the utmost caution. This antibody may be useful in the differentiation of non-small cell carcinomas from small cell carcinomas of the lung. The combined use of anti-TAG-72 and anti-GCDFP-15 is valuable in the diagnosis of apocrine carcinoma.
Content And Storage:
Store at 4C.
Isotype:
IgG1 κ
Antigen: TAG-72
Concentration: 0.2mg/mL
Dilution: Western Blot 1:200-1:400, Flow Cytometry 0.5ug/million cells, ELISA 1-5ug/ml for coating, Immunocytochemistry/Immunofluorescence 1:100-1:200, Immunoprecipitation 1-2ug/500ug protein lysate, Immunohistochemistry-Paraffin 1:200-1:400, Immunohistochemistry-Frozen 1:200-1:400
Host Species: Mouse
Quantity: 0.2 mg
Primary or Secondary: Primary
Target Species: Human, Rat, Bovine, Canine
Form: Purified
Applications: Western Blot, Flow Cytometry, ELISA, Immunocytochemistry, Immunofluorescence, Immunoprecipitation, Immunohistochemistry (Paraffin)
Conjugate: Unconjugated
Formulation: PBS with 0.05% BSA. with 0.05% Sodium Azide
Purification Method: Protein A purified
Regulatory Status: RUO
Test Specificity: Recognizes an oncofetal antigen of 220kDa, identified as a tumor-associated glycoprotein (TAG-72) with properties of a mucin. This MAb defines the mucin-carried sialylated-Tn epitope. TAG-72 is usually expressed by adenocarcinomas, but is negative in mesotheliomas. Studies have reported that this antibody has 80% sensitivity and 93% specificity for pulmonary adenocarcinoma. Therefore, TAG-72 is a useful marker to distinguish between mesothelioma and adenocarcinoma. However, false positive reactions can occur so results must be interpreted with the utmost caution. This antibody may be useful in the differentiation of non-small cell carcinomas from small cell carcinomas of the lung. The combined use of anti-TAG-72 and anti-GCDFP-15 is valuable in the diagnosis of apocrine carcinoma.
Content And Storage: Store at 4C.
Isotype: IgG1 κ
Antigen:
TAG-72
Concentration:
0.2mg/mL
Dilution:
Western Blot 1:200-1:400, Flow Cytometry 0.5ug/million cells, ELISA 1-5ug/ml for coating, Immunocytochemistry/Immunofluorescence 1:100-1:200, Immunoprecipitation 1-2ug/500ug protein lysate, Immunohistochemistry-Paraffin 1:200-1:400, Immunohistochemistry-Frozen 1:200-1:400
Host Species:
Mouse
Quantity:
0.2 mg
Primary or Secondary:
Primary
Target Species:
Human, Rat, Bovine, Canine
Form:
Purified
Applications:
Western Blot, Flow Cytometry, ELISA, Immunocytochemistry, Immunofluorescence, Immunoprecipitation, Immunohistochemistry (Paraffin)
Conjugate:
Unconjugated
Formulation:
PBS with 0.05% BSA. with 0.05% Sodium Azide
Purification Method:
Protein A purified
Regulatory Status:
RUO
Test Specificity:
Recognizes an oncofetal antigen of 220kDa, identified as a tumor-associated glycoprotein (TAG-72) with properties of a mucin. This MAb defines the mucin-carried sialylated-Tn epitope. TAG-72 is usually expressed by adenocarcinomas, but is negative in mesotheliomas. Studies have reported that this antibody has 80% sensitivity and 93% specificity for pulmonary adenocarcinoma. Therefore, TAG-72 is a useful marker to distinguish between mesothelioma and adenocarcinoma. However, false positive reactions can occur so results must be interpreted with the utmost caution. This antibody may be useful in the differentiation of non-small cell carcinomas from small cell carcinomas of the lung. The combined use of anti-TAG-72 and anti-GCDFP-15 is valuable in the diagnosis of apocrine carcinoma.
Content And Storage:
Store at 4C.
Isotype:
IgG1 κ