Mouse IgG1/IgG2a Isotype Control Cocktail, FITC, PE, Invitrogen™

Description

• PA5-33181 is suitable for use as a negative control for the measurement of non-specific binding of mouse monoclonals of isotype IgG1 and IgG2a in human tissues in a dual labelling technique using FITC and R-Phycoerythrin fluorochromes
• This antibody contains a monoclonal mixture of FITC-conjugated Mouse IgG1 RPE-conjugated Mouse IgG2a
• Reconstitute with 0.5 mL of distilled water
• After reconstitution, store product undiluted at 4oC in the dark
• For FACS analysis, use 10 μL of the suggested working dilution to label 1x10^6 cells in 100 μL
• The isotype of a primary antibody and the application it is being used in can result in background staining
• Primary antibody background noise can be caused by binding to Fc receptors on target cells; by non-specific interactions with cellular proteins, carbohydrates, and lipids; or by cell autofluorescence
• Isotype control antibodies can act as negative controls to help differentiate non-specific background signal from specific antibody signal because they have no relevant specificity to a target antigen
• While isotype controls are most commonly used in flow cytometry, they are useful in other applications such as chromatin immunoprecipitation (ChIP), immunohistochemistry, and gel shifts
• Isotype controls should match with the primary antibody species and isotype so that the level of specific staining by the primary antibody may be accurately determined
• If using directly labeled primary antibodies, the isotype control works best if conjugated with the same label as the test antibody.