BDNF Polyclonal Antibody, Invitrogen™

Description

• Reconstitute in 100 μL of sterile water
• Centrifuge to remove any insoluble material
• After reconstitution keep aliquots at -20 °C for a higher stability, and at 4 °C with an appropriate antibacterial agent
• Glycerol (1:1) may be added for an additional stability
• Avoid repetitive freeze/thaw cycles
• BDNF (Brain-Derived Neurotrophic Factor) is a member of the neurotrophin family
• BDNF is synthesized as pre-proBDNF, followed by cleavage to proBDNF
• Although further processing generates the mature, 14 kDa protein, pro-BDNF is biologically active and is secreted in synaptic vesicles along with the mature form
• BDNF is widely expressed in the central nervous system and acts in an autocrine and paracrine manner on several classes of neurons
• Signaling occurs mainly through the tyrosine kinase receptor TrkB, although binding to the lower-affinity receptor, p75-NTR, has also been demonstrated
• BDNF promotes neuronal survival and differentiation and has been shown to play a critical role in memory formation and synaptic regulation
• BDNF is induced by cortical neurons, and is necessary for survival of striatal neurons in the brain
• Expression of BDNF is reduced in both Alzheimer's and Huntington disease patients, and may also play a role in the regulation of stress response and in the biology of mood disorders.