FAS Polyclonal Antibody, Invitrogen™

Description

• Heat-mediated antigen retrieval is recommended prior to staining, using a 10mM citrate buffer, pH 6.0, for 10 minutes followed by cooling at room temperature for 20 min
• Following antigen retrieval, incubate samples with primary antibody for 10 min at room temperature
• A suggested positive control is small intestine or colon tissue
• FAS (Fas, CD95, APO-1) is a 46 kDa transmembrane glycoprotein that functions as a cell death receptor of the TNFR (tumor necrosis factor receptor) superfamily
• FAS also exists in a soluble form that weight approximately 26kDa
• Stimulation of FAS results in aggregation of its intracellular FAS-associated death domains (FADD), formation of the death-inducing signaling complex (DISC) and activation of caspases
• The auto-proteolytic processing of the caspases in the complex trigger a downstream cascade that leads to apoptosis
• FAS has been shown to activate NF-kappaB, MAPK3/ERK1, MAPK8/JNK, and is involved in transducing the proliferating signals in normal diploid fibroblast and T cells
• At least eight alternatively spliced transcript variants encoding seven distinct isoforms have been described
• The isoforms lacking the transmembrane domain may negatively regulate the apoptosis mediated by the full-length isoform
• The Fas/Fas ligand system has been shown to play a role in a number of human diseases such as AIDS, hepatitis and cancer
• It is also assumed that induction of apoptosis through FAS ligand pathway is predominantly involved in anti-viral immune responses.