AFP Monoclonal Antibody (AFP-01), Invitrogen™

Description

• The MA1-19342 anti-alpha Fetoprotein antibody (clone AFP-11) has successfully been paired as the coating antibody in a sandwich ELISA with detection antibody MA1-19178 (clone AFP-01)
• Immunoprecipitation: Interaction of the antibody AFP-01 with AFP is dependent on the presence of calcium ions (strongly inhibited by chelating agents)
• Such characteristics of the antibody can be exploited for immunoaffinity purification of APF under mild elution conditions; Western Blot: non-reducing conditions
• AFP (Alpha Fetoprotein) is a major plasma protein produced by the yolk sac, intestinal tract and the liver during fetal life
• AFP expression in adults is often associated with hepatoma or teratoma
• However, hereditary persistence of AFP may also be found in individuals with no obvious pathology
• The protein is thought to be the fetal counterpart of serum albumin, and AFP and albumin genes are present in tandem in the same transcriptional orientation on chromosome 4
• AFP is found in monomeric, dimeric, and trimeric forms
• AFP can also bind to copper ions, nickel ions, fatty acids and bilirubin
• The level of AFP in amniotic fluid is used to measure renal loss of protein to screen for spina bifida and anencephaly
• High level of serum AFP has been identified in patients with hepatocellular carcinomas (HCC), teratoblastoma, colorectal cancer, pancreatic cancer, and germ cell neoplasms.