05777AF647

MilliporeSigma™ Phosphotyrosine, recombinant, Mouse, Alexa Fluor 647, Clone: 4G10,

Manufacturer: MilliporeSigma™

Select a Size

Pack Size SKU Availability Price
Each of 1 05777AF647-Each-of-1 In Stock ₹ 61,276.36

05777AF647 - Each of 1

₹ 61,276.36

In Stock

Quantity

1

Base Price: ₹ 61,276.36

GST (18%): ₹ 11,029.745

Total Price: ₹ 72,306.105

Antigen

Phosphotyrosine, recombinant

Classification

Monoclonal

Concentration

Please refer to lot specific datasheet.

Formulation

Purified mouse monoclonal IgG2a antibody conjugate in PBS with 15 mg/mL BSA and 0.1 % Sodium Azide.

Immunogen

Produced from CHO cells expressing the 4G10 antibody heavy and light chain cDNAs. Purified via heavy chain C-terminus hexa-histidine tag by Nickel affinity matrices prior to conjugation with Alexa Fluor™ 647.

Quantity

100 μL

Research Discipline

Signaling

Test Specificity

Recognizes tyrosine-phosphorylated proteins from all species.

Content And Storage

Stable for 1 year at 2°-8°C from date of receipt.

Applications

Immunocytochemistry

Clone

4G10

Conjugate

Alexa Fluor 647

Host Species

Mouse

Purification Method

Protein G Purified

Regulatory Status

RUO

Primary or Secondary

Primary

Target Species

All species

Form

Purified

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Description

  • Phosphorylation plays an important role in regulating protein activities and various cellular signaling events
  • Posttranslational phosphorylation can occur on histidine (pHis), serine (pSer), threonine (pThr), and tyrosine (pTyr) residues
  • Due to the early advent of anti-pTyr antibodies, tyrosine phosphorylation remains the mostly studied signaling events in all biological research fields
  • Tyrosine phosphorylation is considered to be one of the key steps in signal transduction and regulation of enzymatic activity
  • Before the availability of anti-pTyr antibodies, protein tyrosine phosphorylation was routiniely detected by time-consuming radioactive labeling
  • Although radioactive labeling is still considered as more sensitive, advancement in detection technology has greatly improved the detection limits of antibody-based methods
  • Anti-pTyr antibodies are commonly used in ELISA, immunoprecipitation, immunoblotting (including dot blot and Western blot), as well as indirect immunofluourescent detection of tyrosine phosphorylation in tissue and cell samples.

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MilliporeSigma™

05777AF647

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Antigen:
Phosphotyrosine, recombinant

Classification:
Monoclonal

Concentration:
Please refer to lot specific datasheet.

Formulation:
Purified mouse monoclonal IgG2a antibody conjugate in PBS with 15 mg/mL BSA and 0.1 % Sodium Azide.

Immunogen:
Produced from CHO cells expressing the 4G10 antibody heavy and light chain cDNAs. Purified via heavy chain C-terminus hexa-histidine tag by Nickel affinity matrices prior to conjugation with Alexa Fluor™ 647.

Quantity:
100 μL

Research Discipline:
Signaling

Test Specificity:
Recognizes tyrosine-phosphorylated proteins from all species.

Content And Storage:
Stable for 1 year at 2°-8°C from date of receipt.

Applications:
Immunocytochemistry

Clone:
4G10

Conjugate:
Alexa Fluor 647

Host Species:
Mouse

Purification Method:
Protein G Purified

Regulatory Status:
RUO

Primary or Secondary:
Primary

Target Species:
All species

Form:
Purified

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Supelco

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