CD44H Monoclonal Antibody (OX49), PE, eBioscience™, Invitrogen™

Description

• Description: This OX49 monoclonal antibody recognizes rat CD44H, which is also known as homing-associated cell adhesion molecule (H-CAM) or phagocytic glycoprotein-1 (Pgp-1)
• This isoform of CD44 is the cell surface receptor for hyaluronate, a component of the extracellular matrix
• CD44H is highly expressed on hematopoietic and non-hematopoietic cells, and exists as multiple splice variants
• CD44H plays a critical role in lymphocyte activation and homing
• Applications Reported: This OX49 antibody has been reported for use in flow cytometric analysis
• Applications Tested: This OX49 antibody has been tested by flow cytometric analysis of rat splenocytes
• This can be used at less than or equal to 0.06 μg per test
• A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL
• Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test
• It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest
• Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser
• Filtration: 0.2 μm post-manufacturing filtered
• CD44 cell surface antigen is a 100kDa glycoprotein widely expressed on human leucocytes, white matter of the brain and by some epithelial cells of the intestine and breast
• Several isoforms of CD44 exist, including the predominant CD44H isoform detected in many normal tissues
• CD44 is a receptor for hyaluronic acid (HA) and is involved in cell-cell interactions, cell adhesion and migration
• CD44 also participates in a wide variety of cellular functions including lymphocyte activation, recirculation and homing
• CD44 expression may be up-regulated upon some carcinomas, and it has been speculated that this may be related to metastatic potential.