CD49b (Integrin alpha 2) Monoclonal Antibody (P1H5), PE, eBioscience™, Invitrogen™

Description

• Description: This P1H5 monoclonal antibody reacts with human CD49b, which is also known as integrin alpha 2
• Integrins constitute a family of cell surface receptors that mediate adhesion between cells or between a cell and the extracellular matrix
• These proteins are heterodimers consisting of two distinct subunits, alpha and beta
• Integrins are expressed on hematopoietic, non-hematopoietic, and cancer cells
• Integrin-mediated adhesion is regulated by changes in ligand affinity (or activation), cell shape, and integrin clustering and diffusion
• Integrin alpha 2 forms a complex with the common beta 1 subunit to form the alpha2beta1 integrin, which binds type I collagen and laminin
• Moreover, studies suggest that this complex suppresses metastasis in cancer and participates in T cell costimulation by activating the MAPK and PI3K pathways
• The P1H5 monoclonal antibody has been reported to inhibit adhesion of fibroblasts and platelets to collagen
• Crossblocking studies indicate that P1H5 and eBioY418 recognize different epitopes
• Applications Reported: This P1H5 antibody has been reported for use in flow cytometric analysis
• Applications Tested: This P1H5 antibody has been pre-diluted and tested by flow cytometric analysis of normal human peripheral blood cells
• This may be used at 5 μL (0.25 μg) per test
• A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL
• CD49b (Integrin alpha 2, Integrin alpha 2 chain, VLA-2 alpha chain, HM alpha 2) is a member of the integrin family
• It is a glycoprotein with molecular weight of 150 kD, and it complexes with CD29 (Integrin beta 1) to form the heterodimeric integrin VLA-2 (integrin alpha 2 beta 1, or GPIa/IIa) complex
• VLA-2 is an extracellular receptor for laminin, collagen, and fibronectin, and interaction with its ligands results in the activation of intracellular signaling pathways
• It has reported roles in VEGF-induced angiogenesis in vivo, as well as adhesion and lymphocyte activation
• CD49b is expressed by NK cells, NK-T cells, monocytes, platelets, and epithelial cells
• It is also expressed on adaptive immune cells such as T and B cells, specifically on a subset of CD4+ T cells in the spleen, on intraepithelial and lamina propria lymphocytes in the intestine, as well as on a population of peripheral CD4+ type 1 T regulatory (Tr1) cells that co-express LAG-3.