CD122 Monoclonal Antibody (5H4), PE, eBioscience™, Invitrogen™

Description

• Description: The 5H4 antibody reacts with mouse CD122, the 90-110 kDa interleukin-2 receptor beta chain
• CD122 is expressed by NK cells and some T cells in the periphery and is upregulated by activation
• CD122 associates with CD25 and CD132 (common gamma chain) to form the high affinity IL-2 receptor
• Binding of IL-2 to CD122 is not inhibited by the 5H4 antibody
• Applications Reported: The 5H4 antibody has been reported for use in flow cytometric analysis
• Applications Tested: The 5H4 antibody has been tested by flow cytometric analysis of resting and activated mouse splenocytes
• This can be used at less than or equal to 0.125 μg per test
• A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL
• Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test
• It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest
• Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser
• Filtration: 0.2 μm post-manufacturing filtered
• CD122 (Interleukin 2 receptor) is involved in T cell-mediated immune responses, primarily expressed in the hematopoietic system and is present in 3 forms
• The low affinity form of CD122 is a monomer of the alpha subunit and is not involved in signal transduction
• The intermediate affinity form consists of an alpha/beta subunit heterodimer, while the high affinity form consists of an alpha/beta/gamma subunit heterotrimer
• Both the intermediate and high affinity forms of the receptor are involved in receptor-mediated endocytosis and transduction of mitogenic signals from CD122
• The protein encoded by the CD122 gene represents the beta subunit and is a type I membrane protein
• Diseases associate with CD122 protein dysfunction include oligoarticular juvenile idiopathic arthritis and rheumatoid factor negative juvenile idiopathic arthritis.