B7-H6 Monoclonal Antibody (JAM1EW), PE, eBioscience™, Invitrogen™

Description

• Description: This JAM1EW monoclonal antibody recognizes human B7-H6 (NCR3LG1) protein on the surface of cells
• It recognizes protein on live but not on paraformaldehyde-fixed cells
• The JAM1EW clone is specific to B7-H6
• When tested in flow cytometry against cells transfected with either B7-H4, B7-H5, B7-H6, or B7-H7, clone JAM1EW only stains positively on B7-H6 transfected cells
• Applications Reported: This JAM1EW antibody has been reported for use in flow cytometric analysis
• Applications Tested: This JAM1EW antibody has been pre-diluted and tested by flow cytometric analysis of HeLa and HL-60 cell line
• This may be used at 5 μL (0.25 μg) per test
• A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL
• Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test
• Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser B7-H6 (NCR3LG1) is a transmembrane endogenous ligand expressed on the surfaces of tumor cells
• B7-H6 binds with NKp30 present on NK cells
• Binding of B7-H6 to NKp30 causes the ligation of NKp30 subsequently inducing NK cell activation and target cell cytolysis
• B7-H6 is not constitutively expressed on normal tissue
• Whether it can be expressed endogenously under certain conditions is under investigation
• B7-H6 has been detected on circulating pro-inflammatory CD14(+)CD16(+) monocytes in some patients with sepsis.