CD370 (Clec9A) Monoclonal Antibody (9A11), PE, eBioscience™, Invitrogen™

Description

• This monoclonal antibody 9A11 reacts with human CD370, also known as C-type lectin family member 9A (Clec9A) and DNGR1
• This type II transmembrane glycoprotein is present at the cell surface in the form of a homodimer
• CD370 is a cellular receptor expressed by myeloid cells that recognizes F-actin, a dead cell-associated antigen that is exposed in necrotic cells
• Upon ligation, CD370 recruits Syk kinase to the ITAM-like motif in its intracellular tail and facilitates endocytosis of antigenic material that is subsequently processed and cross-presented to CD8+ T cells
• Human CD370 is expressed by CD141+ (BDCA3+) dendritic cells and, to a lesser degree, by CD14+ CD16- monocytes
• Unlike in mice, human plasmacytoid dendritic cells do not express CD370
• This 9A11 antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells
• This can be used at 5 μL (0.25 μg) per test
• A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL
• Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test
• Filtration: 0.2 μm post-manufacturing filtered
• CLEC9A is a group V C-type lectin-like receptor (CTLR) that functions as an activation receptor and is expressed on myeloid lineage cells.