E-cadherin Monoclonal Antibody (ECCD-2), Invitrogen™

Description

• Source: Monoclonal antibody was obtained by fusing the P3-X63-Ag8 mouse myeloma cell line with spleen cells of Donryu rat after immunization with mouse teratocarcinoma and mouse liver E-cadherin fragment
• Specificity: This antibody specifically reacts with mouse E-cadherin
• This antibody does not inhibit E-cadherin-dependent cell-cell contact
• Cross reactivity: This antibody reacts with human and dog E-cadherin
• This antibody does not react with bovine or chicken E-cadherin
• Western blot can be performed under reducing or non-reducing conditions
• Reconstitution and dilution: dissolve the lyophilized antibody in 50 μl of distilled water to a final concentration 2.0 mg/mL
• This solution can be used as a stock solution
• If dilution is necessary for your application, dilute the stock solution in 500 μL or more of the following dilution solution: 20mM TBS (pH 7.5), 10mM CaCl2, 1.0% BSA, 0.1% NaN3
• Note that TBS should be used for dilution, as PBS causes precipitation
• Storage: When stored at 4oC, 0.1% sodium azide should be added as a preservative.Store the antibody at a minimum concentration of 2.0 mg/mL; a lower antibody concentration may result in decreased stability.The stock solution (2.0mg/mL) should be stored in aliquots at -20oC for 1year, or stored at 4oC for 6 months after adding 0.1% sodium azide
• Avoid repeated freeze-thaw cycles
• Diluted antibody should not be stored
• E-Cadherin (epithelial cadherin) is a classical cadherin from the cadherin (alcium dependent adhesion protein) superfamily
• The encoded protein is a calcium dependent cell-cell adhesion glycoprotein comprised of five extracellular cadherin repeats, a transmembrane region and a highly conserved cytoplasmic tail
• Mutations in this gene are correlated with gastric, breast, colorectal, thyroid and ovarian cancer
• Loss of function is thought to contribute to progression in cancer by increasing proliferation, invasion, and/or metastasis
• The ectodomain of this protein mediates bacterial adhesion to mammalian cells and the cytoplasmic domain is required for internalization
• Identified transcript variants arise from mutation at consensus splice sites
• E-cadherin plays a central role in the growth and development of cells by controlling tissue architecture, and maintenance of tissue integrity
• In humans, E-cadherin is encoded by the CDH1 gene present on chromosome 16
• Studies have demonstrated that reduction and/or loss of E-cadherin expression in carcinomas correlates positively with the potential of these tumors for invasion and metastasis.