P-cadherin Monoclonal Antibody (PCD-1), Invitrogen™

Description

• Source: Monoclonal antibody was obtained by fusing the P3-X63-Ag8-U1 mouse myeloma cell-line with spleen cells of Donryu rat after immunization with mouse endoderm cell line PSA5-E
• Specificity: This antibody specifically reacts with mouse P-cadherin
• This antibody inhibits P-cadherin-dependent cell-cell contact
• Cross reactivity: This antibody does not react with human or chicken P-cadherin
• Storage: 4°C This product does not contain preservative
• The stock solution (2.0 mg/ml) should be stored in aliquots at -20°C for 1 year, or should be stored at 4°C for 6 months after adding 0.1% sodium azide
• Avoid repeated freeze-thaw cycles
• Reconstitution: Dissolve the lyophilized antibody in 50 μl of distilled water (final concentration: 2.0 mg/ml)
• This solution can be used as a stock solution
• If dilution is necessary for your application, dilute the stock solution with the following Dilution solution just prior to use
• When the entire amount of antibody is to be used over a short time period, it may be dissolved directly in 500 μl or more of the Dilution solution
• Be sure to store the antibody at a minimum concentration of 2.0 mg/ml
• A lower antibody concentration may result in decreased stability
• Reconstituted antibody solution should contain 0.1% sodium azide as a preservative when stored at 4°C
• Ca2+ should be contained in the buffers to stabilize cadherin antigen
• P-cadherin, also known as Cadherin-3, is a Type 1 cadherin protein that belongs to the cadherin superfamily
• Type 1 cadherins are single-pass transmembrane proteins that have 5 extracellular cadherin repeats and an intracellular domain that binds p120-catenin and beta-catenin
• Cadherins are calcium-dependent cell-cell adhesion glycoproteins responsible for a range of processes including development, wound healing, cell-cell signaling, cell growth and differentiation
• P-cadherin is expressed in human fetal structures, and in adult tissues such as the basal layer of the epidermis, breast, prostate, ovary, cervix, hair follicle, and corneal endothelium
• P-cadherin expression has also been reported on embryonic stem cells, and stem cells of the normal mammary gland, and hair follicle
• Overexpression of P-cadherin has been associated with poor prognosis in breast, prostate, ovary, colon, and stomach carcinomas
• Mutations in CDH3 are associated with congential hypotrichosis with juvenile macular dystrophy.