STAT5 alpha Monoclonal Antibody (ST5a-2H2), Invitrogen™

Description

• This antibody recognizes the STAT5a protein from a variety of species including human, mouse, and rat and it reacts specifically with the STAT5a protein and does not exhibit any cross-reactivity with the highly related STAT5b protein
• Cross-reactivity with other endogenous STAT isoforms has not been observed
• Reactivity has been confirmed by western blotting using cell lysates derived from human A431 cells (+/- EGF treatment), NIH 3T3 cells, MCF-7 cells, and HeLa cells
• STAT5 alpha is a member of the STAT family of transcription factors
• In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators
• STAT5 alpha is activated by, and mediates the responses of many cell ligands, such as IL2, IL3, IL7 GM-CSF, erythropoietin, thrombopoietin, and different growth hormones
• Activation of STAT5 alpha in myeloma and lymphoma associated with a TEL/JAK2 gene fusion is independent of cell stimulus and has been shown to be essential for the tumorigenesis
• The mouse counterpart of STAT5 alpha is found to induce the expression of BCL2L1/BCL-X(L), which suggests the antiapoptotic function of this protein in cells
• STAT5 alpha, along with STAT5 beta, were identified in mouse
• The amino acid sequences of STAT5 alpha and STAT5 beta show 96% sequence similarity, and both proteins are co expressed in most tissues in virgin and lactating mice
• However, differential accumulation of STAT5 alpha and STAT5 beta mRNA has been reported for both muscle and mammary tissue
• STAT5 alpha is critically involved in a variety of physiological functions, including reproduction, lactation, immune function and somatic growth.