APC Monoclonal Antibody (eBioAPC-6A2), Biotin, eBioscience™, Invitrogen™

Description

• Description: The eBioAPC-6A2 monoclonal antibody reacts with allophycocyanin (APC) and APC-Cy7, with no crossreactivity to fluorescein (FITC) or phycoerythrin (PE)
• When using eBioAPC-6A2 as a second-step antibody, in combination with an appropriate third step, to bind a APC-conjugated primary antibody, there is no decrease in the fluorescent intensity of the directly conjugated APC antibody
• Therefore no interference or quenching of the APC fluorochrome is observed
• The eBioAPC-6A2 can be used for separation of cells labeled with APC-conjugated antibodies or for staining
• Please note that the optimal concentration for this antibody will vary depending on the density of the primary APC-conjugated antibody and the application tested
• Applications Reported: This eBioAPC-6A2 antibody has been reported for use in flow cytometric analysis
• Applications Tested: This eBioAPC-6A2 antibody has been tested by flow cytometric analysis of normal human peripheral blood cells stained with an APC-conjugated antibody
• This can be used at less than or equal to 0.015 μg per test
• A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL
• Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test
• It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest
• Filtration: 0.2 μm post-manufacturing filtered
• Anti-APC (Allophycocyanin) antibodies can be used to bind APC-conjugated primary antibodies without decreasing fluorescent intensity of the APC fluorochrome
• This method is used for separation of cells labeled with APC-conjugated antibodies.