alpha-Fetoprotein Monoclonal Antibody (1E8), Biotin, eBioscience™, Invitrogen™

Description

• Description: This 1E8 monoclonal antibody reacts with human alpha-fetoprotein (AFP)
• This 70-kDa secretory protein is a member of the albumin gene family
• Synthesized by the yolk sac and fetal liver during embryogenesis, AFP protein levels are highest in fetal serum
• After birth, serum AFP levels decrease dramatically
• In fact, AFP is nearly undetectable in normal adult serum
• However, hepatocellular carcinoma and germ cell teratoblastoma, as well as liver regeneration, viral hepatitis, and cirrhosis, leads to elevated AFP serum levels in adults
• As such, detection of this protein is frequently used as a diagnostic marker for these conditions
• Applications Reported: This 1E8 antibody has been reported for use in microscopy, immunohistochemical staining, and immunocytochemistry
• Applications Tested: This 1E8 antibody has been tested by immunohistochemistry on formalin-fixed paraffin embedded tissue using high pH antigen retrieval at 20 μg/mL
• It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest
• Filtration: 0.2 μm post-manufacturing filtered
• AFP (Alpha Fetoprotein) is a major plasma protein produced by the yolk sac, intestinal tract and the liver during fetal life
• AFP expression in adults is often associated with hepatoma or teratoma
• However, hereditary persistence of AFP may also be found in individuals with no obvious pathology
• The protein is thought to be the fetal counterpart of serum albumin, and AFP and albumin genes are present in tandem in the same transcriptional orientation on chromosome 4
• AFP is found in monomeric, dimeric, and trimeric forms
• AFP can also bind to copper ions, nickel ions, fatty acids and bilirubin
• The level of AFP in amniotic fluid is used to measure renal loss of protein to screen for spina bifida and anencephaly
• High level of serum AFP has been identified in patients with hepatocellular carcinomas (HCC), teratoblastoma, colorectal cancer, pancreatic cancer, and germ cell neoplasms.