CD62P (P-Selectin) Monoclonal Antibody (AK-4), eBioscience™, Invitrogen™

Description

• Description: The AK-4 monoclonal antibody reacts with human CD62P, a 140 kDa transmembrane glycoprotein also known as P-Selectin
• Upon activation, CD62P is rapidly transported from its cytoplasmic stores in the Weibel-Palade bodies of endothelial cells or the alpha-granules of platelets to the surface
• CD62P initiates the adhesive interaction between endothelial cells and neutrophils and monocytes during the inflammatory reaction and is also involved in the interaction of platelets with monocytes and neutrophils
• Applications Reported: The AK-4 antibody has been reported for use in flow cytometric analysis, and immunohistochemical staining of frozen tissue sections
• AK-4 has also been reported in blocking adhesion of platelets in in vitro assays
• Applications Tested: This AK-4 antibody has been tested by flow cytometric analysis of resting and thrombin-activated human platelets
• This can be used at less than or equal to 1 μg per test
• A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL
• Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test
• It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest
• Purity: Greater than 90%, as determined by SDS-PAGE
• Aggregation: Less than 10%, as determined by HPLC
• Filtration: 0.2 μm post-manufacturing filtered
• P-selectin (Selectin P, GMP-140, LECAM3, CD62 antigen-like family member) is a 140 kDa type 1 transmembrane glycoprotein, and is one of the most commonly studied proteins that regulate cell rolling
• P-selectin is stored in the Weibel-Palade bodies of endothelial cells, as well as in a-granules of platelets
• From there, it can be rapidly brought to the cell surface after exposure to thrombin, histamine, complement 5a, Ca21 ionophores, or adenosine diphosphate
• P-selectin protein redistributes to the plasma membrane during platelet activation and degranulation and mediates the interaction of activated endothelial cells or platelets with leukocytes
• P-selectin plays an important role in adhesive processes between leucocytes and endothelial cells, and is a calcium dependent receptor that binds to sialylated forms of Lewis blood group carbohydrate antigens found on neutrophils and monocytes
• P-selectin is constitutively expressed in inflammation and contributes to atherogenesis, thrombosis and tissue destruction
• Clinically, P-selectin is used to distinguish heparin induced thrombocytopenia with and without thrombosis.