CD79b Monoclonal Antibody (CB3-1), eBioscience™, Invitrogen™

Description

• Description: This CB3-1 monoclonal antibody reacts with human CD79b, the B cell antigen receptor Ig-beta chain
• Together with the Ig-alpha chain, also known as CD79a, this cell surface molecule forms a heterodimeric complex that associates with membrane immunoglobulin (mIgM)
• CD79b expression is exclusive to B lymphocytes and B cell lymphomas
• Depending on the maturation state of the B cell, CD79b can be expressed either in the cytoplasm or on the cell surface
• The CD79 receptor complex triggers numerous signaling pathways to mediate B cell development, maintenance, and activation
• Applications Reported: This CB3-1 antibody has been reported for use in flow cytometric analysis and immunoblotting (WB)
• Applications Tested: This CB3-1 antibody has been tested by flow cytometric analysis of normal human peripheral blood cells
• This can be used at less than or equal to 0.5 μg per test
• A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL
• Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test
• It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest
• Purity: Greater than 90%, as determined by SDS-PAGE
• Aggregation: Less than 10%, as determined by HPLC
• Filtration: 0.2 μm post-manufacturing filtered
• CD79b is the B cell antigen receptor Ig beta chain (BCR-Ig beta), which associates with B cell antigen receptor Ig alpha chain to form a functional heterodimer that interacts with membrane immunoglobulin (mIg)
• The mIgM component binds antigen and the Ig alpha /I g beta heterodimer tranduces signals.