Staphylococcus aureus Cas9 Monoclonal Antibody (6H4), eBioscience™, Invitrogen™

Description

• Description: This 6H4 monoclonal antibody recognizes Staphylococcus aureus Cas9 endonuclease
• Applications Reported: This 6H4 antibody has been reported for use in immunocytochemical staining and western blot
• (Fluorochrome-conjugated 6H4 is recommended for use in flow cytometery.) Applications Tested: This 6H4 antibody has been tested by immunocytochemical staining of Staphylococcus aureus derived Cas9 transfected A549 cells
• This may be used at less than or equal to 0.6 μg/mL
• This 6H4 antibody has been tested by western blot of Staphylococcus aureus derived Cas9 transfected A549 cells
• This may be used at less than or equal to 4 μg/mL detecting a band at 124 kDa
• Cas9 is an RNA-guided DNA endonuclease enzyme associated with the CRISPR (Clustered Regularly Interspaced Short Palindromic Repeats) adaptive immunity system in bacteria and genetic engineering
• The system is unique and flexible due to its dependence on RNA as the element that targets the nuclease to a desired DNA sequence
• It can be used to induce indel mutations, specific sequence replacements/insertions, and large deletions or genomic rearrangements at any desired location in the genome
• In addition, Cas9 can also be used to mediate upregulation of specific endogenous genes or to alter histone modifications or DNA methylation
• Cas9 may be derived from Streptococcus pyogenes (SpCas9) or Staphylococcus aureus (SaCas9)
• SaCas9 has a number of properties that make it advantageous for genome editing, including its small size, high efficiency, nickase activity, and apparent specificity
• SaCas9 principally recognizes an NNGRRT protospacer adjacent motif (PAM) and cleaves target DNA at rates comparable to SpCas9 with an ideal spacer lengths of 24 to 20 nucleotides
• In comparison to SpCas9, SaCas9 is smaller (1368 AA versus 1053 AA) and generates indels at a comparable rate when directed against target DNA with the mutually permissive NGGRRT PAM
• This makes it the preferred enzyme for all-in-one delivery of Cas9 and multiple gRNA expression cassettes with Adeno-associated vectors.