IL-12 p35 Monoclonal Antibody (C18.2), eBioscience™, Invitrogen™

Description

• The C18.2 antibody reacts with the p35 subunit of mouse interleukin-12 (IL-12)
• Interleukin-12 (IL-12) is a heterodimeric 70 kD (p70) cytokine composed of two covalently linked, glycosylated chains, 40kD (p40) and 35-kD (p35)
• IL-12 is mainly produced by monocytes, macrophages, and dendritic cells in response to bacterial products such as lipopolysaccharides (LPS), to intracellular pathogens or upon interaction with activated T cells
• IL-12 was originally discovered because of its ability to induce interferon-gamma (IFN-g) production, cell proliferation, and cytotoxicity mediated by natural killer cells and T cells
• It is now established that IL-12 also plays a key role in the development of Th1 responses, leading to IFN-g and IL-2 production
• These cytokines can in turn promote T-cell responses and macrophage activation
• Interleukin 12A is a novel signaling cytokine belonging to the Interleukin-6 protein superfamily
• Interleukin-12 alpha chain precursor (IL-12A) is a 35-kD subunit of disulfide-linked heterodimer of cytokine IL-12 and has a broad array of biological activities
• Reports suggest that IL-12A can act as a growth factor for activated T and NK cells and enhance the lytic activity of NK/lymphokine-activated Killer cells
• IL-12A stimulates the production of IFN-gamma by resting PBMC as well as T-cell-independent induction of interferon (IFN)-gamma, and helps in the differentiation of both Th1 and Th2 cells
• It is also involved in positive regulation of tyrosine phosphorylation of Stat4 protein
• The signaling process of IL-12A in innate immunity requires nitric oxide synthase 2A
• Along with EBI3, IL-12A forms a novel heterodimeric cytokine, designated as interleukin-35 (IL-35), that may be specifically produced by T reg cells and is required for maximal suppressive activity.