CD4 Monoclonal Antibody (GK1.5), Functional Grade, eBioscience™, Invitrogen™

Description

• The GK1.5 MAb reacts with the mouse CD4 molecule, a 55 kDa cell surface receptor expressed by a majority of thymocytes, subpopulation of mature T cells and dendritic cells
• CD4 binds to MHC class II on the surface of antigen presenting cells and plays an important role both in T cell development and in optimal functioning of mature T cells
• In T cells, CD4 associates with protein tyrosine kinase p56lck through its cytoplasmic tail
• Binding of GK1.5 is blocked by RM4-5
• Applications Reported: The GK1.5 antibody has been reported for use in flow cytometric analysis
• It has also been reported in isolation and depletion of CD4+ cells, blocking of ligand binding and T cell activation via CD4
• Applications Tested: The GK1.5 antibody has been tested by flow cytometric analysis of mouse thymocytes and splenocytes
• This can be used at less than or equal to 0.125 μg per test
• A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL
• Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test
• It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest
• Storage and handling: Use in a sterile environment
• Filtration: 0.2 μm post-manufacturing filtered
• Purity: Greater than 90%, as determined by SDS-PAGE
• Endotoxin Level: Less than 0.001 ng/ μg antibody, as determined by LAL assay
• Aggregation: Less than 10%, as determined by HPLC
• The CD4 antigen is involved in the recognition of MHC class II molecules and is a co-receptor for HIV
• CD4 is primarily expressed in a subset of T-lymphocytes, also referred to as T helper cells, but may also be expressed by other cells in the immune system, such as monocytes, macrophages, and dendritic cells
• At the tissue level, CD4 expression may be detected in thymus, lymph nodes, tonsils, and spleen, and also in specific regions of the brain, gut, and other non-lymphoid tissues
• CD4 functions to initiate or augment the early phase of T-cell activation through its association with the T-cell receptor complex and protein tyrosine kinase, Lck
• It may also function as an important mediator of direct neuronal damage in infectious and immune-mediated diseases of the central nervous system
• Multiple alternatively spliced transcripts have been identified in this gene [RefSeq, July 2017].