CD117 (c-Kit) Monoclonal Antibody (ACK2), Functional Grade, eBioscience™, Invitrogen™

Description

• The ACK2 MAb reacts with mouse CD117, also known as c-Kit receptor, Steel factor receptor and stem cell factor receptor
• A member of the tyrosine kinase receptor family, this 145 kDa molecule is expressed by a majority of hematopoietic progenitor cells characterized in the mouse bone marrow as a small subset of cells positive for Sca-1 and Thy1 (Thy1^lo) and negative for lineage markers
• The interaction of the mouse c-kit receptor and steel factor promotes the proliferation and differentiation of hematopoietic progenitor cells
• CD117 is also expressed by mast cells and plays a role in signaling and activation of these cells
• ACK2 has been reported to be a blocking antibody
• KIT (c-KIT) is a proto-oncogene and a type 3 transmembrane receptor for MGF (mast cell growth factor, also known as stem cell factor)
• KIT was first identified as the cellular homolog of the feline sarcoma viral oncogene v-kit
• KIT together with its ligand regulates growth and activation of a variety of hemopoietic and non-hemopoietic cells
• Mutations in KIT are associated with gastrointestinal stromal tumors, mast cell disease, acute myelogenous leukemia, and piebaldism
• Recently, deregulation of the KIT receptor TK by the prevalent activation loop mutation D816V has served as a focal point in therapeutic strategies aimed at curbing neoplastic mast cell growth
• c-Kit is expressed in hematopoietic stem cells, germ cells, mast cells and gastrointestinal tract cajal cells
• Upon binding of its ligand stem cell factor (SCF), c-kit dimerizes, resulting in receptor activation and autophosphorylation of various tyrosine residues including tyrosine 703 located on the cytoplasmic domain of the receptor
• This modification allows docking of Grb2 and activation of the Ras/ERK signaling pathway
• SCF/c-kit can activate multiple downstream signaling pathways including PI3K, PLC-gamma and JAK/STAT
• c-kit receptor activation is essential for hematopoiesis, stem cell maintenance and gametogenesis.