HLA-E Monoclonal Antibody (3D12HLA-E), Functional Grade, eBioscience™, Invitrogen™

Description

• Description: The monoclonal antibody 3D12 (HLA-E) recognizes human HLA-E, a member of the Human Leukocyte Antigen family
• HLA-E is a MHC Class IB member, which has a specialized role in NK cell recognition
• Binding of a restricted set of peptides derived from other Class I leader sequences to the HLA-E and B2M (beta2 microglobulin) complex leads to interaction with NKG2A on NK cells
• This binding protects the HLA-G-expressing cell from NK cell killing
• HLA-E expression is ubiquitous
• Applications Reported: This 3D12HLA-E antibody has been reported for use in flow cytometric analysis
• Applications Tested: This 3D12HLA-E antibody has been tested by flow cytometric analysis of normal human peripheral blood cells
• This can be used at less than or equal to 0.5 μg per test
• A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL
• Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test
• It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest
• Storage and handling: Use in a sterile environment
• Filtration: 0.2 μm post-manufacturing filtered
• Purity: Greater than 90%, as determined by SDS-PAGE
• Endotoxin Level: Less than 0.001 ng/μg antibody, as determined by LAL assay
• Aggregation: Less than 10%, as determined by HPLC
• HLA-E belongs to the HLA class I heavy chain paralogues
• This class I molecule is a heterodimer consisting of a heavy chain and a light chain (beta-2 microglobulin)
• The heavy chain is anchored in the membrane
• HLA-E binds a restricted subset of peptides derived from the leader peptides of other class I molecules
• The heavy chain is approximately 45 kDa and its gene contains 8 exons
• Exon one encodes the leader peptide, exons 2 and 3 encode the alpha1 and alpha2 domains, which both bind the peptide, exon 4 encodes the alpha3 domain, exon 5 encodes the transmembrane region, and exons 6 and 7 encode the cytoplasmic tail
• HLA and MHC antibodies play a significant role in Immunopeptidomics, facilitating the identification and characterization of neoantigens through high-performance liquid chromatography coupled to tandem Mass Spectrometry.