CD43 Monoclonal Antibody (4-29-5-10-21), APC, eBioscience™, Invitrogen™

Description

• Description: The monoclonal antibody 4-29-5-10-21 reacts with human and non-human primate CD43, which is also known as leukosialin, galactoglycoprotein and sialophorin
• CD43 is a sialomucin which, like many mucins, can have both adhesive and anti-adhesive functions
• Expression of CD43 is found on most leukocytes and a small subset of B lymphocytes
• Proteolytic processing upon activation decreases surface expression
• CD43 is involved in the activation of T cells, B cells, NK cells, and monocytes
• The counter-receptor for CD43 is CD169/SIGLEC-1, which is expressed on macrophages
• Applications Reported: This 4-29-5-10-21 antibody has been reported for use in flow cytometric analysis
• Applications Tested: This 4-29-5-10-21 antibody has been pre-titrated and tested by flow cytometric anaylsis of normal human peripheral blood cells
• This can be used at 5 μL (0.125 μg) per test
• A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL
• Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test
• Excitation: 633-647 nm; Emission: 660 nm; Laser: Red Laser
• CD43 (leukosialin, sialophorin) is a transmembrane mucin-like protein with high negative charge, expressed on the surface of most hematopoietic cells
• CD43 contributes to a repulsive barrier that interferes with cellular adhesion, however, in certain cases also promotes leukocyte aggregation
• By interaction with actin-binding proteins ezrin and moesin CD43 plays a regulatory role in remodeling T-cell morphology and regulates cell-cell interactions during lymphocyte traffic
• CD43 signaling both enhances LFA-1 adhesiveness and counteracts LFA-1 induction via other receptors
• Expression of CD43 causes induction of functionally active tumor suppressor p53 protein, but in case of p53 and ARF deficiency CD43 promotes tumor proliferation and viability
• CD43 appears to be an important modulator of leukocyte functions
• Diseases associated with CD43 dysfunction include Wiscott-Aldrich Syndrome and Adenoid Basal Cell Carcinoma.