CD49d (Integrin alpha 4) Monoclonal Antibody (MRa4-1), APC, eBioscience™, Invitrogen™

Description

• Description: The MRa4-1 monoclonal antibody reacts with CD49d, which is also known as the integrin alpha 4 subunit
• This 150-kDa cell surface protein is expressed by thymocytes, peripheral lymphocytes, monocytes, and eosinophils in complex with either integrin beta1 or integrin beta7
• Outside of the immune system, CD49d is also expressed on mast cells, neural crest cells, retinal cells, and dorsal root ganglion neurons
• Integrins are responsible for lymphocyte homing to Peyer's patches and mesenteric lymph nodes, as well as neural development and regeneration
• Several ligands have been reported for the integrin a4b1 complex, including VCAM-1 (CD106) and fibronectin
• The MRa1-4 antibody has been reported to inhibit fibronectin binding and lymphocyte migration
• Applications Reported: This MRa4-1 antibody has been reported for use in flow cytometric analysis
• Applications Tested: This MRa4-1 antibody has been tested by flow cytometric analysis of rat splenocytes
• This can be used at less than or equal to 0.5 μg per test
• A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL
• Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test
• It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest
• Excitation: 633-647 nm; Emission: 660 nm; Laser: Red Laser
• Filtration: 0.2 μm post-manufacturing filtered
• The integrin alpha 4 (also known as CD49d and ITGA4) belongs to the integrin alpha chain family of proteins
• Integrins are heterodimeric integral membrane proteins composed of an alpha and beta chains
• Alpha4 chain associates with either beta1 or beta7 chain
• It has been demonstrated that the putative ligand-binding sites of both integrin alpha4beta1 and alpha4beta7 is located on the alpha4 chain
• These ligands included Madcam, VCAM, and fibronectin
• Madcam is known as the principal ligand for integrin alpha4beta7
• Recently it was also demonstrated that HIV-1 envelope can mimic Madcam by binding to and signaling though integrin alpha4beta7, the gut mucosal homing receptor for peripheral T cells.